Show simple item record

dc.contributor.authorLesche, D
dc.contributor.authorGeyer, R
dc.contributor.authorLienhard, D
dc.contributor.authorNakas, CT
dc.contributor.authorDiserens, G
dc.contributor.authorVermathen, P
dc.contributor.authorLeichtle, AB
dc.date.accessioned2021-02-04T02:12:37Z
dc.date.available2021-02-04T02:12:37Z
dc.date.issued2016-10-01
dc.identifierpii: 1109
dc.identifier.citationLesche, D., Geyer, R., Lienhard, D., Nakas, C. T., Diserens, G., Vermathen, P. & Leichtle, A. B. (2016). Does centrifugation matter? Centrifugal force and spinning time alter the plasma metabolome. METABOLOMICS, 12 (10), https://doi.org/10.1007/s11306-016-1109-3.
dc.identifier.issn1573-3882
dc.identifier.urihttp://hdl.handle.net/11343/259609
dc.description.abstractBACKGROUND: Centrifugation is an indispensable procedure for plasma sample preparation, but applied conditions can vary between labs. AIM: Determine whether routinely used plasma centrifugation protocols (1500×g 10 min; 3000×g 5 min) influence non-targeted metabolomic analyses. METHODS: Nuclear magnetic resonance spectroscopy (NMR) and High Resolution Mass Spectrometry (HRMS) data were evaluated with sparse partial least squares discriminant analyses and compared with cell count measurements. RESULTS: Besides significant differences in platelet count, we identified substantial alterations in NMR and HRMS data related to the different centrifugation protocols. CONCLUSION: Already minor differences in plasma centrifugation can significantly influence metabolomic patterns and potentially bias metabolomics studies.
dc.languageEnglish
dc.publisherSPRINGER
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleDoes centrifugation matter? Centrifugal force and spinning time alter the plasma metabolome
dc.typeJournal Article
dc.identifier.doi10.1007/s11306-016-1109-3
melbourne.affiliation.departmentSir Peter MacCallum Department of Oncology
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titleMetabolomics
melbourne.source.volume12
melbourne.source.issue10
dc.rights.licenseCC BY
melbourne.elementsid1220088
melbourne.contributor.authorLesche, Dorothea
dc.identifier.eissn1573-3890
melbourne.accessrightsOpen Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record