University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Medical School
  • Obstetrics and Gynaecology
  • Obstetrics and Gynaecology - Research Publications
  • View Item
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Medical School
  • Obstetrics and Gynaecology
  • Obstetrics and Gynaecology - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    Increased methylation and decreased expression of homeobox genes TLX1, HOXA10 and DLX5 in human placenta are associated with trophoblast differentiation

    Thumbnail
    Download
    Published version (2.750Mb)

    Citations
    Scopus
    Altmetric
    8
    Author
    Novakovic, B; Fournier, T; Harris, LK; James, J; Roberts, CT; Yong, HEJ; Kalionis, B; Evain-Brion, D; Ebeling, PR; Wallace, EM; ...
    Date
    2017-07-03
    Source Title
    Scientific Reports
    Publisher
    NATURE PUBLISHING GROUP
    University of Melbourne Author/s
    Kalionis, Bill; Murthi, Padma; Saffery, Richard; Yong, Ee
    Affiliation
    Obstetrics and Gynaecology
    Paediatrics (RCH)
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Novakovic, B., Fournier, T., Harris, L. K., James, J., Roberts, C. T., Yong, H. E. J., Kalionis, B., Evain-Brion, D., Ebeling, P. R., Wallace, E. M., Saffery, R. & Murthi, P. (2017). Increased methylation and decreased expression of homeobox genes TLX1, HOXA10 and DLX5 in human placenta are associated with trophoblast differentiation. SCIENTIFIC REPORTS, 7 (1), https://doi.org/10.1038/s41598-017-04776-5.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/259610
    DOI
    10.1038/s41598-017-04776-5
    NHMRC Grant code
    NHMRC/509140
    Abstract
    Homeobox genes regulate embryonic and placental development, and are widely expressed in the human placenta, but their regulatory control by DNA methylation is unclear. DNA methylation analysis was performed on human placentae from first, second and third trimesters to determine methylation patterns of homeobox gene promoters across gestation. Most homeobox genes were hypo-methylated throughout gestation, suggesting that DNA methylation is not the primary mechanism involved in regulating HOX genes expression in the placenta. Nevertheless, several genes showed variable methylation patterns across gestation, with a general trend towards an increase in methylation over gestation. Three genes (TLX1, HOXA10 and DLX5) showed inverse gains of methylation with decreasing mRNA expression throughout pregnancy, supporting a role for DNA methylation in their regulation. Proteins encoded by these genes were primarily localised to the syncytiotrophoblast layer, and showed decreased expression later in gestation. siRNA mediated downregulation of DLX5, TLX1 and HOXA10 in primary term villous cytotrophoblast resulted in decreased proliferation and increased expression of differentiation markers, including ERVW-1. Our data suggest that loss of DLX5, TLX1 and HOXA10 expression in late gestation is required for proper placental differentiation and function.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [52369]
    • Paediatrics (RCH) - Research Publications [2382]
    • Obstetrics and Gynaecology - Research Publications [828]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors