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    Induced Pluripotent Stem Cell Derived Macrophages as a Cellular System to Study Salmonella and Other Pathogens

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    26
    Author
    Hale, C; Yeung, A; Goulding, D; Pickard, D; Alasoo, K; Powrie, F; Dougan, G; Mukhopadhyay, S
    Date
    2015-05-06
    Source Title
    PLoS One
    Publisher
    PUBLIC LIBRARY SCIENCE
    University of Melbourne Author/s
    Dougan, Gordon
    Affiliation
    Microbiology and Immunology
    Metadata
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    Document Type
    Journal Article
    Citations
    Hale, C., Yeung, A., Goulding, D., Pickard, D., Alasoo, K., Powrie, F., Dougan, G. & Mukhopadhyay, S. (2015). Induced Pluripotent Stem Cell Derived Macrophages as a Cellular System to Study Salmonella and Other Pathogens. PLOS ONE, 10 (5), https://doi.org/10.1371/journal.pone.0124307.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/259986
    DOI
    10.1371/journal.pone.0124307
    Abstract
    A number of pathogens, including several human-restricted organisms, persist and replicate within macrophages (Mφs) as a key step in pathogenesis. The mechanisms underpinning such host-restricted intracellular adaptations are poorly understood, in part, due to a lack of appropriate model systems. Here we explore the potential of human induced pluripotent stem cell derived macrophages (iPSDMs) to study such pathogen interactions. We show iPSDMs express a panel of established Mφ-specific markers, produce cytokines, and polarise into classical and alternative activation states in response to IFN-γ and IL-4 stimulation, respectively. iPSDMs also efficiently phagocytosed inactivated bacterial particles as well as live Salmonella Typhi and S. Typhimurium and were able to kill these pathogens. We conclude that iPSDMs can support productive Salmonella infection and propose this as a flexible system to study host/pathogen interactions. Furthermore, iPSDMs can provide a flexible and practical cellular platform for assessing host responses in multiple genetic backgrounds.

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