Locating and Activating Molecular 'Time Bombs': Induction of Mycolata Prophages
AuthorDyson, ZA; Brown, TL; Farrar, B; Doyle, SR; Tucci, J; Seviour, RJ; Petrovski, S
Source TitlePLoS One
PublisherPUBLIC LIBRARY SCIENCE
University of Melbourne Author/sDyson, Zoe
AffiliationBiochemistry and Molecular Biology
Document TypeJournal Article
CitationsDyson, Z. A., Brown, T. L., Farrar, B., Doyle, S. R., Tucci, J., Seviour, R. J. & Petrovski, S. (2016). Locating and Activating Molecular 'Time Bombs': Induction of Mycolata Prophages. PLOS ONE, 11 (8), https://doi.org/10.1371/journal.pone.0159957.
Access StatusOpen Access
Little is known about the prevalence, functionality and ecological roles of temperate phages for members of the mycolic acid producing bacteria, the Mycolata. While many lytic phages infective for these organisms have been isolated, and assessed for their suitability for use as biological control agents of activated sludge foaming, no studies have investigated how temperate phages might be induced for this purpose. Bioinformatic analysis using the PHAge Search Tool (PHAST) on Mycolata whole genome sequence data in GenBank for members of the genera Gordonia, Mycobacterium, Nocardia, Rhodococcus, and Tsukamurella revealed 83% contained putative prophage DNA sequences. Subsequent prophage inductions using mitomycin C were conducted on 17 Mycolata strains. This led to the isolation and genome characterization of three novel Caudovirales temperate phages, namely GAL1, GMA1, and TPA4, induced from Gordonia alkanivorans, Gordonia malaquae, and Tsukamurella paurometabola, respectively. All possessed highly distinctive dsDNA genome sequences.
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