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dc.contributor.authorHill, CB
dc.contributor.authorCassin, A
dc.contributor.authorKeeble-Gagnere, G
dc.contributor.authorDoblin, MS
dc.contributor.authorBacic, A
dc.contributor.authorRoessner, U
dc.date.accessioned2021-02-05T00:27:55Z
dc.date.available2021-02-05T00:27:55Z
dc.date.issued2016-08-16
dc.identifierpii: srep31558
dc.identifier.citationHill, C. B., Cassin, A., Keeble-Gagnere, G., Doblin, M. S., Bacic, A. & Roessner, U. (2016). De novo transcriptome assembly and analysis of differentially expressed genes of two barley genotypes reveal root-zone-specific responses to salt exposure. SCIENTIFIC REPORTS, 6 (1), https://doi.org/10.1038/srep31558.
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11343/260016
dc.description.abstractPlant roots are the first organs sensing and responding to salinity stress, manifested differentially between different root types, and also at the individual tissue and cellular level. High genetic diversity and the current lack of an assembled map-based sequence of the barley genome severely limit barley research potential. We used over 580 and 600 million paired-end reads, respectively, to create two de novo assemblies of a barley landrace (Sahara) and a malting cultivar (Clipper) with known contrasting responses to salinity. Generalized linear models were used to statistically access spatial, treatment-related, and genotype-specific responses. This revealed a spatial gene expression gradient along the barley root, with more differentially expressed transcripts detected between different root zones than between treatments. The root transcriptome also showed a gradual transition from transcripts related to sugar-mediated signaling at the root meristematic zone to those involved in cell wall metabolism in the elongation zone, and defense response-related pathways toward the maturation zone, with significant differences between the two genotypes. The availability of these additional transcriptome reference sets will serve as a valuable resource to the cereal research community, and may identify valuable traits to assist in breeding programmes.
dc.languageEnglish
dc.publisherNATURE PUBLISHING GROUP
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleDe novo transcriptome assembly and analysis of differentially expressed genes of two barley genotypes reveal root-zone-specific responses to salt exposure
dc.typeJournal Article
dc.identifier.doi10.1038/srep31558
melbourne.affiliation.departmentSchool of BioSciences
melbourne.affiliation.facultyScience
melbourne.source.titleScientific Reports
melbourne.source.volume6
melbourne.source.issue1
dc.rights.licenseCC BY
melbourne.elementsid1092532
melbourne.contributor.authorCassin, Andrew
melbourne.contributor.authorDoblin, Monika
melbourne.contributor.authorBacic, Anthony
melbourne.contributor.authorRoessner, Ute
melbourne.contributor.authorHill, Camilla
melbourne.contributor.authorKeeble-Gagnere, Gabriel
dc.identifier.eissn2045-2322
melbourne.accessrightsOpen Access


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