Interaction of Salmonella Typhimurium with Dendritic Cells Derived from Pluripotent Embryonic Stem Cells

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Rossi, R; Hale, C; Goulding, D; Andrews, R; Abdellah, Z; Fairchild, PJ; Dougan, GDate
2012-12-28Source Title
PLoS OnePublisher
PUBLIC LIBRARY SCIENCEUniversity of Melbourne Author/s
Dougan, GordonAffiliation
Microbiology and ImmunologyMetadata
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Journal ArticleCitations
Rossi, R., Hale, C., Goulding, D., Andrews, R., Abdellah, Z., Fairchild, P. J. & Dougan, G. (2012). Interaction of Salmonella Typhimurium with Dendritic Cells Derived from Pluripotent Embryonic Stem Cells. PLOS ONE, 7 (12), https://doi.org/10.1371/journal.pone.0052232.Access Status
Open AccessAbstract
Using an in vitro differentiation protocol we isolated cells with the properties of dendritic cells (DCs) from immunologically refractive pluripotent murine embryonic stem cells (ESCs). These ES-derived dendritic cells (ESDCs) expressed cytokines and were able to present antigen to a T cell line. Infection of ESDCs with Salmonella Typhimurium stimulated the expression of immune cell markers and thousands of murine genes, many associated with the immune response. Consequently, this system provides a novel in vitro model, amenable to genetic modification, for monitoring host/pathogen interactions.
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