Interaction of Salmonella Typhimurium with Dendritic Cells Derived from Pluripotent Embryonic Stem Cells

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    Author
    Rossi, R; Hale, C; Goulding, D; Andrews, R; Abdellah, Z; Fairchild, PJ; Dougan, G
    Date
    2012-12-28
    Source Title
    PLoS One
    Publisher
    PUBLIC LIBRARY SCIENCE
    University of Melbourne Author/s
    Dougan, Gordon
    Affiliation
    Microbiology and Immunology
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    Document Type
    Journal Article
    Citations
    Rossi, R., Hale, C., Goulding, D., Andrews, R., Abdellah, Z., Fairchild, P. J. & Dougan, G. (2012). Interaction of Salmonella Typhimurium with Dendritic Cells Derived from Pluripotent Embryonic Stem Cells. PLOS ONE, 7 (12), https://doi.org/10.1371/journal.pone.0052232.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/260022
    DOI
    10.1371/journal.pone.0052232
    Abstract
    Using an in vitro differentiation protocol we isolated cells with the properties of dendritic cells (DCs) from immunologically refractive pluripotent murine embryonic stem cells (ESCs). These ES-derived dendritic cells (ESDCs) expressed cytokines and were able to present antigen to a T cell line. Infection of ESDCs with Salmonella Typhimurium stimulated the expression of immune cell markers and thousands of murine genes, many associated with the immune response. Consequently, this system provides a novel in vitro model, amenable to genetic modification, for monitoring host/pathogen interactions.

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