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dc.contributor.authorDuncan, LM
dc.contributor.authorTimms, RT
dc.contributor.authorZavodszky, E
dc.contributor.authorCano, F
dc.contributor.authorDougan, G
dc.contributor.authorRandow, F
dc.contributor.authorLehner, PJ
dc.date.accessioned2021-02-05T00:29:21Z
dc.date.available2021-02-05T00:29:21Z
dc.date.issued2012-06-22
dc.identifierpii: PONE-D-12-14658
dc.identifier.citationDuncan, L. M., Timms, R. T., Zavodszky, E., Cano, F., Dougan, G., Randow, F. & Lehner, P. J. (2012). Fluorescence-Based Phenotypic Selection Allows Forward Genetic Screens in Haploid Human Cells. PLOS ONE, 7 (6), https://doi.org/10.1371/journal.pone.0039651.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11343/260026
dc.description.abstractThe isolation of haploid cell lines has recently allowed the power of forward genetic screens to be applied to mammalian cells. The interest in applying this powerful genetic approach to a mammalian system is only tempered by the limited utility of these screens, if confined to lethal phenotypes. Here we expand the scope of these approaches beyond live/dead screens and show that selection for a cell surface phenotype via fluorescence-activated cell sorting can identify the key molecules in an intracellular pathway, in this case MHC class I antigen presentation. Non-lethal haploid genetic screens are widely applicable to identify genes involved in essentially any cellular pathway.
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleFluorescence-Based Phenotypic Selection Allows Forward Genetic Screens in Haploid Human Cells
dc.typeJournal Article
dc.identifier.doi10.1371/journal.pone.0039651
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titlePLoS One
melbourne.source.volume7
melbourne.source.issue6
dc.rights.licenseCC BY
melbourne.elementsid1080658
melbourne.contributor.authorDougan, Gordon
dc.identifier.eissn1932-6203
melbourne.accessrightsOpen Access


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