University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Veterinary and Agricultural Sciences
  • Agriculture and Food Systems
  • Agriculture and Food Systems - Research Publications
  • View Item
  • Minerva Access
  • Veterinary and Agricultural Sciences
  • Agriculture and Food Systems
  • Agriculture and Food Systems - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    Multi-environment QTL studies suggest a role for cysteine-rich protein kinase genes in quantitative resistance to blackleg disease in Brassica napus

    Thumbnail
    Download
    Published version (1.520Mb)

    Citations
    Scopus
    Altmetric
    29
    Author
    Larkan, NJ; Raman, H; Lydiate, DJ; Robinson, SJ; Yu, F; Barbulescu, DM; Raman, R; Luckett, DJ; Burton, W; Wratten, N; ...
    Date
    2016-08-24
    Source Title
    BMC Plant Biology
    Publisher
    BIOMED CENTRAL LTD
    University of Melbourne Author/s
    Salisbury, Phillip
    Affiliation
    Agriculture and Food Systems
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Larkan, N. J., Raman, H., Lydiate, D. J., Robinson, S. J., Yu, F., Barbulescu, D. M., Raman, R., Luckett, D. J., Burton, W., Wratten, N., Salisbury, P. A., Rimmer, S. R. & Borhan, M. H. (2016). Multi-environment QTL studies suggest a role for cysteine-rich protein kinase genes in quantitative resistance to blackleg disease in Brassica napus. BMC PLANT BIOLOGY, 16 (1), https://doi.org/10.1186/s12870-016-0877-2.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/260091
    DOI
    10.1186/s12870-016-0877-2
    Abstract
    BACKGROUND: Resistance to the blackleg disease of Brassica napus (canola/oilseed rape), caused by the hemibiotrophic fungal pathogen Leptosphaeria maculans, is determined by both race-specific resistance (R) genes and quantitative resistance loci (QTL), or adult-plant resistance (APR). While the introgression of R genes into breeding material is relatively simple, QTL are often detected sporadically, making them harder to capture in breeding programs. For the effective deployment of APR in crop varieties, resistance QTL need to have a reliable influence on phenotype in multiple environments and be well defined genetically to enable marker-assisted selection (MAS). RESULTS: Doubled-haploid populations produced from the susceptible B. napus variety Topas and APR varieties AG-Castle and AV-Sapphire were analysed for resistance to blackleg in two locations over 3 and 4 years, respectively. Three stable QTL were detected in each population, with two loci appearing to be common to both APR varieties. Physical delineation of three QTL regions was sufficient to identify candidate defense-related genes, including a cluster of cysteine-rich receptor-like kinases contained within a 49 gene QTL interval on chromosome A01. Individual L. maculans isolates were used to define the physical intervals for the race-specific R genes Rlm3 and Rlm4 and to identify QTL common to both field studies and the cotyledon resistance response. CONCLUSION: Through multi-environment QTL analysis we have identified and delineated four significant and stable QTL suitable for MAS of quantitative blackleg resistance in B. napus, and identified candidate genes which potentially play a role in quantitative defense responses to L. maculans.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [52443]
    • Agriculture and Food Systems - Research Publications [655]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors