University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Medical School
  • Paediatrics (RCH)
  • Paediatrics (RCH) - Research Publications
  • View Item
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Medical School
  • Paediatrics (RCH)
  • Paediatrics (RCH) - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    Polarity, cell division, and out-of-equilibrium dynamics control the growth of epithelial structures

    Thumbnail
    Download
    Published version (2.636Mb)

    Citations
    Scopus
    Altmetric
    27
    Author
    Cerruti, B; Puliafito, A; Shewan, AM; Yu, W; Combes, AN; Little, MH; Chianale, F; Primo, L; Serini, G; Mostov, KE; ...
    Date
    2013-10-28
    Source Title
    The Journal of Cell Biology
    Publisher
    ROCKEFELLER UNIV PRESS
    University of Melbourne Author/s
    Combes, Alexander; Little, Melissa
    Affiliation
    Paediatrics (RCH)
    Anatomy and Neuroscience
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Cerruti, B., Puliafito, A., Shewan, A. M., Yu, W., Combes, A. N., Little, M. H., Chianale, F., Primo, L., Serini, G., Mostov, K. E., Celani, A. & Gamba, A. (2013). Polarity, cell division, and out-of-equilibrium dynamics control the growth of epithelial structures. JOURNAL OF CELL BIOLOGY, 203 (2), pp.359-372. https://doi.org/10.1083/jcb.201305044.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/260099
    DOI
    10.1083/jcb.201305044
    Abstract
    The growth of a well-formed epithelial structure is governed by mechanical constraints, cellular apico-basal polarity, and spatially controlled cell division. Here we compared the predictions of a mathematical model of epithelial growth with the morphological analysis of 3D epithelial structures. In both in vitro cyst models and in developing epithelial structures in vivo, epithelial growth could take place close to or far from mechanical equilibrium, and was determined by the hierarchy of time-scales of cell division, cell-cell rearrangements, and lumen dynamics. Equilibrium properties could be inferred by the analysis of cell-cell contact topologies, and the nonequilibrium phenotype was altered by inhibiting ROCK activity. The occurrence of an aberrant multilumen phenotype was linked to fast nonequilibrium growth, even when geometric control of cell division was correctly enforced. We predicted and verified experimentally that slowing down cell division partially rescued a multilumen phenotype induced by altered polarity. These results improve our understanding of the development of epithelial organs and, ultimately, of carcinogenesis.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [52443]
    • Anatomy and Neuroscience - Research Publications [804]
    • Paediatrics (RCH) - Research Publications [2383]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors