Show simple item record

dc.contributor.authorCerruti, B
dc.contributor.authorPuliafito, A
dc.contributor.authorShewan, AM
dc.contributor.authorYu, W
dc.contributor.authorCombes, AN
dc.contributor.authorLittle, MH
dc.contributor.authorChianale, F
dc.contributor.authorPrimo, L
dc.contributor.authorSerini, G
dc.contributor.authorMostov, KE
dc.contributor.authorCelani, A
dc.contributor.authorGamba, A
dc.date.accessioned2021-02-05T00:39:47Z
dc.date.available2021-02-05T00:39:47Z
dc.date.issued2013-10-28
dc.identifierpii: jcb.201305044
dc.identifier.citationCerruti, B., Puliafito, A., Shewan, A. M., Yu, W., Combes, A. N., Little, M. H., Chianale, F., Primo, L., Serini, G., Mostov, K. E., Celani, A. & Gamba, A. (2013). Polarity, cell division, and out-of-equilibrium dynamics control the growth of epithelial structures. JOURNAL OF CELL BIOLOGY, 203 (2), pp.359-372. https://doi.org/10.1083/jcb.201305044.
dc.identifier.issn0021-9525
dc.identifier.urihttp://hdl.handle.net/11343/260099
dc.description.abstractThe growth of a well-formed epithelial structure is governed by mechanical constraints, cellular apico-basal polarity, and spatially controlled cell division. Here we compared the predictions of a mathematical model of epithelial growth with the morphological analysis of 3D epithelial structures. In both in vitro cyst models and in developing epithelial structures in vivo, epithelial growth could take place close to or far from mechanical equilibrium, and was determined by the hierarchy of time-scales of cell division, cell-cell rearrangements, and lumen dynamics. Equilibrium properties could be inferred by the analysis of cell-cell contact topologies, and the nonequilibrium phenotype was altered by inhibiting ROCK activity. The occurrence of an aberrant multilumen phenotype was linked to fast nonequilibrium growth, even when geometric control of cell division was correctly enforced. We predicted and verified experimentally that slowing down cell division partially rescued a multilumen phenotype induced by altered polarity. These results improve our understanding of the development of epithelial organs and, ultimately, of carcinogenesis.
dc.languageEnglish
dc.publisherROCKEFELLER UNIV PRESS
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0
dc.titlePolarity, cell division, and out-of-equilibrium dynamics control the growth of epithelial structures
dc.typeJournal Article
dc.identifier.doi10.1083/jcb.201305044
melbourne.affiliation.departmentPaediatrics (RCH)
melbourne.affiliation.departmentAnatomy and Neuroscience
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titleThe Journal of Cell Biology
melbourne.source.volume203
melbourne.source.issue2
melbourne.source.pages359-372
dc.rights.licenseCC BY-NC-SA
melbourne.elementsid958833
melbourne.contributor.authorCombes, Alexander
melbourne.contributor.authorLittle, Melissa
dc.identifier.eissn1540-8140
melbourne.accessrightsOpen Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record