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dc.contributor.authorPereira, LA
dc.contributor.authorHugo, HJ
dc.contributor.authorMalaterre, J
dc.contributor.authorXu, H
dc.contributor.authorSonza, S
dc.contributor.authorCures, A
dc.contributor.authorPurcell, DFJ
dc.contributor.authorRamsland, PA
dc.contributor.authorGerondakis, S
dc.contributor.authorGonda, TJ
dc.contributor.authorRamsay, RG
dc.date.accessioned2021-02-05T00:43:05Z
dc.date.available2021-02-05T00:43:05Z
dc.date.issued2015-04-08
dc.identifierpii: PONE-D-14-26281
dc.identifier.citationPereira, L. A., Hugo, H. J., Malaterre, J., Xu, H., Sonza, S., Cures, A., Purcell, D. F. J., Ramsland, P. A., Gerondakis, S., Gonda, T. J. & Ramsay, R. G. (2015). MYB Elongation Is Regulated by the Nucleic Acid Binding of NF kappa B p50 to the Intronic Stem-Loop Region. PLOS ONE, 10 (4), https://doi.org/10.1371/journal.pone.0122919.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11343/260122
dc.description.abstractMYB transcriptional elongation is regulated by an attenuator sequence within intron 1 that has been proposed to encode a RNA stem loop (SLR) followed by a polyU tract. We report that NFκBp50 can bind the SLR polyU RNA and promote MYB transcriptional elongation together with NFκBp65. We identified a conserved lysine-rich motif within the Rel homology domain (RHD) of NFκBp50, mutation of which abrogated the interaction of NFκBp50 with the SLR polyU and impaired NFκBp50 mediated MYB elongation. We observed that the TAR RNA-binding region of Tat is homologous to the NFκBp50 RHD lysine-rich motif, a finding consistent with HIV Tat acting as an effector of MYB transcriptional elongation in an SLR dependent manner. Furthermore, we identify the DNA binding activity of NFκBp50 as a key component required for the SLR polyU mediated regulation of MYB. Collectively these results suggest that the MYB SLR polyU provides a platform for proteins to regulate MYB and reveals novel nucleic acid binding properties of NFκBp50 required for MYB regulation.
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.titleMYB Elongation Is Regulated by the Nucleic Acid Binding of NF kappa B p50 to the Intronic Stem-Loop Region
dc.typeJournal Article
dc.identifier.doi10.1371/journal.pone.0122919
melbourne.affiliation.departmentSir Peter MacCallum Department of Oncology
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.affiliation.departmentSurgery (Austin & Northern Health)
melbourne.affiliation.departmentClinical Pathology
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titlePLoS One
melbourne.source.volume10
melbourne.source.issue4
melbourne.identifier.nhmrc400216
melbourne.identifier.nhmrc400231
dc.rights.licenseCC BY
melbourne.elementsid968440
melbourne.contributor.authorMALATERRE, JORDANE
melbourne.contributor.authorSonza, Secondo
melbourne.contributor.authorPurcell, Damian
melbourne.contributor.authorRamsland, Paul
melbourne.contributor.authorPereira, Lloyd
melbourne.contributor.authorXu, Huiling
melbourne.contributor.authorRamsay, Robert
dc.identifier.eissn1932-6203
melbourne.identifier.fundernameidNHMRC, 400216
melbourne.identifier.fundernameidNHMRC, 400231
melbourne.identifier.fundernameidWALTER AND ELIZA HALL INSTITUTE OF MEDICAL RESEARCH, 487922
melbourne.accessrightsOpen Access


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