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dc.contributor.authorParoly, SS
dc.contributor.authorWang, F
dc.contributor.authorSpraggon, L
dc.contributor.authorMerregaert, J
dc.contributor.authorBatourina, E
dc.contributor.authorTycko, B
dc.contributor.authorSchmidt-Ott, KM
dc.contributor.authorGrimmond, S
dc.contributor.authorLittle, M
dc.contributor.authorMendelsohn, C
dc.date.accessioned2021-02-05T00:44:15Z
dc.date.available2021-02-05T00:44:15Z
dc.date.issued2013-12-31
dc.identifierpii: PONE-D-13-35748
dc.identifier.citationParoly, S. S., Wang, F., Spraggon, L., Merregaert, J., Batourina, E., Tycko, B., Schmidt-Ott, K. M., Grimmond, S., Little, M. & Mendelsohn, C. (2013). Stromal Protein Ecm1 Regulates Ureteric Bud Patterning and Branching. PLOS ONE, 8 (12), https://doi.org/10.1371/journal.pone.0084155.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11343/260129
dc.description.abstractThe interactions between the nephrogenic mesenchyme and the ureteric bud during kidney development are well documented. While recent studies have shed some light on the importance of the stroma during renal development, many of the signals generated in the stroma, the genetic pathways and interaction networks involving the stroma are yet to be identified. Our previous studies demonstrate that retinoids are crucial for branching of the ureteric bud and for patterning of the cortical stroma. In the present study we demonstrate that autocrine retinoic acid (RA) signaling in stromal cells is critical for their survival and patterning, and show that Extracellular matrix 1, Ecm1, a gene that in humans causes irritable bowel syndrome and lipoid proteinosis, is a novel RA-regulated target in the developing kidney, which is secreted from the cortical stromal cells surrounding the cap mesenchyme and ureteric bud. Our studies suggest that Ecm1 is required in the ureteric bud for regulating the distribution of Ret which is normally restricted to the tips, as inhibition of Ecm1 results in an expanded domain of Ret expression and reduced numbers of branches. We propose a model in which retinoid signaling in the stroma activates expression of Ecm1, which in turn down-regulates Ret expression in the ureteric bud cleft, where bifurcation normally occurs and normal branching progresses.
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleStromal Protein Ecm1 Regulates Ureteric Bud Patterning and Branching
dc.typeJournal Article
dc.identifier.doi10.1371/journal.pone.0084155
melbourne.affiliation.departmentPaediatrics (RCH)
melbourne.affiliation.departmentCentre for Cancer Research
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titlePLoS One
melbourne.source.volume8
melbourne.source.issue12
dc.rights.licenseCC BY
melbourne.elementsid1033272
melbourne.contributor.authorLittle, Melissa
melbourne.contributor.authorGrimmond, Sean
dc.identifier.eissn1932-6203
melbourne.accessrightsOpen Access


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