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dc.contributor.authorLeong, TL
dc.contributor.authorMarini, KD
dc.contributor.authorRossello, FJ
dc.contributor.authorJayasekara, SN
dc.contributor.authorRussell, PA
dc.contributor.authorProdanovic, Z
dc.contributor.authorKumar, B
dc.contributor.authorGanju, V
dc.contributor.authorAlamgeer, M
dc.contributor.authorIrving, LB
dc.contributor.authorSteinfort, DP
dc.contributor.authorPeacock, CD
dc.contributor.authorCain, JE
dc.contributor.authorSzczepny, A
dc.contributor.authorWatkins, DN
dc.date.accessioned2021-02-05T00:52:49Z
dc.date.available2021-02-05T00:52:49Z
dc.date.issued2014-09-05
dc.identifierpii: PONE-D-14-25801
dc.identifier.citationLeong, T. L., Marini, K. D., Rossello, F. J., Jayasekara, S. N., Russell, P. A., Prodanovic, Z., Kumar, B., Ganju, V., Alamgeer, M., Irving, L. B., Steinfort, D. P., Peacock, C. D., Cain, J. E., Szczepny, A. & Watkins, D. N. (2014). Genomic Characterisation of Small Cell Lung Cancer Patient-Derived Xenografts Generated from Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration Specimens. PLOS ONE, 9 (9), https://doi.org/10.1371/journal.pone.0106862.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11343/260189
dc.description.abstractPatient-derived xenograft (PDX) models generated from surgical specimens are gaining popularity as preclinical models of cancer. However, establishment of PDX lines from small cell lung cancer (SCLC) patients is difficult due to very limited amount of available biopsy material. We asked whether SCLC cells obtained from endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) could generate PDX lines that maintained the phenotypic and genetic characteristics of the primary tumor. Following successful EBUS-TBNA sampling for diagnostic purposes, we obtained an extra sample for cytologic analysis and implantation into the flanks of immunodeficient mice. Animals were monitored for engraftment for up to 6 months. Histopathologic and immunohistochemical analysis, and targeted next-generation re-sequencing, were then performed in both the primary sample and the derivative PDX line. A total of 12 patients were enrolled in the study. EBUS-TBNA aspirates yielded large numbers of viable tumor cells sufficient to inject between 18,750 and 1,487,000 cells per flank, and to yield microgram quantities of high-quality DNA. Of these, samples from 10 patients generated xenografts (engraftment rate 83%) with a mean latency of 104 days (range 63-188). All but one maintained a typical SCLC phenotype that closely matched the original sample. Identical mutations that are characteristic of SCLC were identified in both the primary sample and xenograft line. EBUS-TBNA has the potential to be a powerful tool in the development of new targeting strategies for SCLC patients by providing large numbers of viable tumor cells suitable for both xenografting and complex genomic analysis.
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleGenomic Characterisation of Small Cell Lung Cancer Patient-Derived Xenografts Generated from Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration Specimens
dc.typeJournal Article
dc.identifier.doi10.1371/journal.pone.0106862
melbourne.affiliation.departmentAnatomy and Neuroscience
melbourne.affiliation.departmentClinical Pathology
melbourne.affiliation.departmentMedicine (Austin & Northern Health)
melbourne.affiliation.departmentMedicine (RMH)
melbourne.affiliation.departmentRadiology
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titlePLoS One
melbourne.source.volume9
melbourne.source.issue9
dc.rights.licenseCC BY
melbourne.elementsid1036901
melbourne.contributor.authorIrving, Louis
melbourne.contributor.authorSteinfort, Daniel
melbourne.contributor.authorRussell, Prudence
melbourne.contributor.authorLeong, Tracy Li-Tsein
melbourne.contributor.authorRossello, Fernando
melbourne.contributor.authorLeong, Tracy Li-Tsein
dc.identifier.eissn1932-6203
melbourne.accessrightsOpen Access


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