University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Medical School
  • Biochemistry and Molecular Biology
  • Biochemistry and Molecular Biology - Research Publications
  • View Item
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Medical School
  • Biochemistry and Molecular Biology
  • Biochemistry and Molecular Biology - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    iSRAP - a one-touch research tool for rapid profiling of small RNA-seq data

    Thumbnail
    Download
    Published version (1.748Mb)

    Citations
    Scopus
    Altmetric
    10
    Author
    Quek, C; Jung, C-H; Bellingham, SA; Lonie, A; Hill, AF
    Date
    2015-01-01
    Source Title
    Journal of Extracellular Vesicles
    Publisher
    TAYLOR & FRANCIS LTD
    University of Melbourne Author/s
    Hill, Andrew; Lonie, Andrew; Bellingham, Shayne; Jung, Chol-Hee
    Affiliation
    Biochemistry and Molecular Biology
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Quek, C., Jung, C. -H., Bellingham, S. A., Lonie, A. & Hill, A. F. (2015). iSRAP - a one-touch research tool for rapid profiling of small RNA-seq data. JOURNAL OF EXTRACELLULAR VESICLES, 4 (1), https://doi.org/10.3402/jev.v4.29454.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/260203
    DOI
    10.3402/jev.v4.29454
    NHMRC Grant code
    NHMRC/628946
    NHMRC/628946
    NHMRC/628946
    NHMRC/628946
    NHMRC/628946
    NHMRC/628946
    NHMRC/628946
    ARC Grant code
    ARC/FT100100560
    Abstract
    Small non-coding RNAs have been significantly recognized as the key modulators in many biological processes, and are emerging as promising biomarkers for several diseases. These RNA species are transcribed in cells and can be packaged in extracellular vesicles, which are small vesicles released from many biotypes, and are involved in intercellular communication. Currently, the advent of next-generation sequencing (NGS) technology for high-throughput profiling has further advanced the biological insights of non-coding RNA on a genome-wide scale and has become the preferred approach for the discovery and quantification of non-coding RNA species. Despite the routine practice of NGS, the processing of large data sets poses difficulty for analysis before conducting downstream experiments. Often, the current analysis tools are designed for specific RNA species, such as microRNA, and are limited in flexibility for modifying parameters for optimization. An analysis tool that allows for maximum control of different software is essential for drawing concrete conclusions for differentially expressed transcripts. Here, we developed a one-touch integrated small RNA analysis pipeline (iSRAP) research tool that is composed of widely used tools for rapid profiling of small RNAs. The performance test of iSRAP using publicly and in-house available data sets shows its ability of comprehensive profiling of small RNAs of various classes, and analysis of differentially expressed small RNAs. iSRAP offers comprehensive analysis of small RNA sequencing data that leverage informed decisions on the downstream analyses of small RNA studies, including extracellular vesicles such as exosomes.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [52369]
    • Biochemistry and Molecular Biology - Research Publications [1066]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors