Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen.

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    Author
    Bannai, H; Nemoto, M; Tsujimura, K; Yamanaka, T; Maeda, K; Kondo, T
    Date
    2016-02
    Source Title
    Journal of Veterinary Medical Science
    Publisher
    Japanese Society of Veterinary Science
    University of Melbourne Author/s
    Bannai, Hiroshi
    Affiliation
    Veterinary Biosciences
    Metadata
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    Document Type
    Journal Article
    Citations
    Bannai, H., Nemoto, M., Tsujimura, K., Yamanaka, T., Maeda, K. & Kondo, T. (2016). Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen.. J Vet Med Sci, 78 (2), pp.309-311. https://doi.org/10.1292/jvms.15-0275.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/260206
    DOI
    10.1292/jvms.15-0275
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4785124
    Abstract
    To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4 (EHV-4) that uses a 12-mer peptide of glycoprotein G (gG4-12-mer: MKNNPIYSEGSL) [4], we used a longer peptide consisting of a 24-mer repeat sequence (gG4-24-mer: MKNNPIYSEGSLMLNVQHDDSIHT) as an antigen. Sera of horses experimentally infected with EHV-4 reacted much more strongly to the gG4-24-mer peptide than to the gG4-12-mer peptide. We used peptide ELISAs to test paired sera from horses naturally infected with EHV-4 (n=40). gG4-24-mer ELISA detected 37 positive samples (92.5%), whereas gG4-12-mer ELISA detected only 28 (70.0%). gG4-24-mer ELISA was much more sensitive than gG4-12-mer ELISA.

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