University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Medical School
  • Surgery (St Vincent's)
  • Surgery (St Vincent's) - Research Publications
  • View Item
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Medical School
  • Surgery (St Vincent's)
  • Surgery (St Vincent's) - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    Chondrogenesis of Human Infrapatellar Fat Pad Stem Cells on Acellular Dermal Matrix

    Thumbnail
    Download
    Published version (1.893Mb)

    Citations
    Scopus
    Altmetric
    6
    Author
    Ye, K; Traianedes, K; Choong, PFM; Myers, DE
    Date
    2016-01-26
    Source Title
    Frontiers in Surgery
    Publisher
    FRONTIERS MEDIA SA
    University of Melbourne Author/s
    Choong, Peter; Myers, Damian
    Affiliation
    Surgery (St Vincent's)
    Medicine and Radiology
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Ye, K., Traianedes, K., Choong, P. F. M. & Myers, D. E. (2016). Chondrogenesis of Human Infrapatellar Fat Pad Stem Cells on Acellular Dermal Matrix. FRONTIERS IN SURGERY, 3, https://doi.org/10.3389/fsurg.2016.00003.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/260228
    DOI
    10.3389/fsurg.2016.00003
    NHMRC Grant code
    NHMRC/1017633
    Abstract
    Acellular dermal matrix (ADM) has been in clinical use for decades in numerous surgical applications. The ability for ADM to promote cellular repopulation, revascularisation and tissue regeneration is well documented. Adipose stem cells have the ability to differentiate into mesenchymal tissue types, including bone and cartilage. The aim of this study was to investigate the potential interaction between ADM and adipose stem cells in vitro using TGFβ3 and BMP6. Human infrapatellar fat pad-derived adipose stem cells (IPFP-ASC) were cultured with ADM derived from rat dermis in chondrogenic (TGFβ3 and BMP6) medium in vitro for 2 and 4 weeks. Histology, qPCR, and immunohistochemistry were performed to assess for markers of chondrogenesis (collagen Type II, SOX9 and proteoglycans). At 4 weeks, cell-scaffold constructs displayed cellular changes consistent with chondrogenesis, with evidence of stratification of cell layers and development of a hyaline-like cartilage layer superficially, which stained positively for collagen Type II and proteoglycans. Significant cell-matrix interaction was seen between the cartilage layer and the ADM itself with seamless integration between each layer. Real time qPCR showed significantly increased COL2A1, SOX9, and ACAN gene expression over 4 weeks when compared to control. COL1A2 gene expression remained unchanged over 4 weeks. We believe that the principles that make ADM versatile and successful for tissue regeneration are applicable to cartilage regeneration. This study demonstrates in vitro the ability for IPFP-ASCs to undergo chondrogenesis, infiltrate, and interact with ADM. These outcomes serve as a platform for in vivo modelling of ADM for cartilage repair.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [52369]
    • Medicine and Radiology - Research Publications [3309]
    • Surgery (St Vincent's) - Research Publications [431]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors