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dc.contributor.authorShelden, MC
dc.contributor.authorDias, DA
dc.contributor.authorJayasinghe, NS
dc.contributor.authorBacic, A
dc.contributor.authorRoessner, U
dc.date.accessioned2021-02-05T00:59:51Z
dc.date.available2021-02-05T00:59:51Z
dc.date.issued2016-06-01
dc.identifierpii: erw059
dc.identifier.citationShelden, M. C., Dias, D. A., Jayasinghe, N. S., Bacic, A. & Roessner, U. (2016). Root spatial metabolite profiling of two genotypes of barley (Hordeum vulgare L.) reveals differences in response to short-term salt stress. JOURNAL OF EXPERIMENTAL BOTANY, 67 (12), pp.3731-3745. https://doi.org/10.1093/jxb/erw059.
dc.identifier.issn0022-0957
dc.identifier.urihttp://hdl.handle.net/11343/260235
dc.description.abstractBarley (Hordeum vulgare L.) is the most salt-tolerant cereal crop and has excellent genetic and genomic resources. It is therefore a good model to study salt-tolerance mechanisms in cereals. We aimed to determine metabolic differences between a cultivated barley, Clipper (tolerant), and a North African landrace, Sahara (susceptible), previously shown to have contrasting root growth phenotypes in response to the early phase of salinity stress. GC-MS was used to determine spatial changes in primary metabolites in barley roots in response to salt stress, by profiling three different regions of the root: root cap/cell division zone (R1), elongation zone (R2), and maturation zone (R3). We identified 76 known metabolites, including 29 amino acids and amines, 20 organic acids and fatty acids, and 19 sugars and sugar phosphates. The maintenance of cell division and root elongation in Clipper in response to short-term salt stress was associated with the synthesis and accumulation of amino acids (i.e. proline), sugars (maltose, sucrose, xylose), and organic acids (gluconate, shikimate), indicating a potential role for these metabolic pathways in salt tolerance and the maintenance of root elongation. The processes involved in root growth adaptation and the underlying coordination of metabolic pathways appear to be controlled in a region-specific manner. This study highlights the importance of utilizing spatial profiling and will provide us with a better understanding of abiotic stress response(s) in plants at the tissue and cellular level.
dc.languageEnglish
dc.publisherOXFORD UNIV PRESS
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleRoot spatial metabolite profiling of two genotypes of barley (Hordeum vulgare L.) reveals differences in response to short-term salt stress
dc.typeJournal Article
dc.identifier.doi10.1093/jxb/erw059
melbourne.affiliation.departmentSchool of BioSciences
melbourne.affiliation.facultyScience
melbourne.source.titleJournal of Experimental Botany
melbourne.source.volume67
melbourne.source.issue12
melbourne.source.pages3731-3745
dc.rights.licenseCC BY
melbourne.elementsid1042001
melbourne.contributor.authorRoessner, Ute
melbourne.contributor.authorDias, Daniel
melbourne.contributor.authorJayasinghe, Nirupama
melbourne.contributor.authorBacic, Anthony
dc.identifier.eissn1460-2431
melbourne.accessrightsOpen Access


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