Show simple item record

dc.contributor.authorGopal, SK
dc.contributor.authorGreening, DW
dc.contributor.authorHanssen, EG
dc.contributor.authorZhu, H-J
dc.contributor.authorSimpson, RJ
dc.contributor.authorMathias, RA
dc.date.accessioned2021-02-05T01:03:11Z
dc.date.available2021-02-05T01:03:11Z
dc.date.issued2016-04-12
dc.identifierpii: 7573
dc.identifier.citationGopal, S. K., Greening, D. W., Hanssen, E. G., Zhu, H. -J., Simpson, R. J. & Mathias, R. A. (2016). Oncogenic epithelial cell-derived exosomes containing Rac1 and PAK2 induce angiogenesis in recipient endothelial cells. ONCOTARGET, 7 (15), pp.19709-19722. https://doi.org/10.18632/oncotarget.7573.
dc.identifier.issn1949-2553
dc.identifier.urihttp://hdl.handle.net/11343/260253
dc.description.abstractThe metastatic cascade describes the escape of primary tumour cells to distant secondary sites. Cells at the leading tumour edge are thought to undergo epithelial-mesenchymal transition (EMT), to enhance their motility and invasion for spreading. Whether EMT cells directly promote tumour angiogenesis, and the role of exosomes (30-150 nm extracellular vesicles) remains largely unknown. We examined the functional effects of exosomes from MDCK cells, MDCK cells stably expressing YBX1 (MDCKYBX1, intermediate EMT), and Ras-transformed MDCK cells (21D1 cells, complete EMT). 2F-2B cell motility and tube formation (length and branching) was significantly increased following supplementation with MDCKYBX1 or 21D1 exosomes, but not MDCK exosomes. Next, Matrigel™ plugs containing exosome-supplemented 2F-2B cells were subcutaneously injected into mice. Systemic perfusion was only observed for plugs supplemented with MDCKYBX1 or 21D1 exosomes. Comparative proteomics revealed that 21D1 exosomes contained VEGF-associated proteins, while MDCKYBX1 exosomes were enriched with activated Rac1 and PAK2. To validate, 2F-2B cells and HUVECs were pre-treated with PAK inhibitors prior to exosome supplementation. PAK inhibition nullified the effects of MDCKYBX1 exosomes by reducing the tube length and branching to baseline levels. By contrast, the effects of 21D1 exosomes were not significantly decreased. Our results demonstrate for the first time that oncogenic cells undergoing EMT can communicate with endothelial cells via exosomes, and establish exosomal Rac1/PAK2 as angiogenic promoters that may function from early stages of the metastatic cascade.
dc.languageEnglish
dc.publisherIMPACT JOURNALS LLC
dc.titleOncogenic epithelial cell-derived exosomes containing Rac1 and PAK2 induce angiogenesis in recipient endothelial cells
dc.typeJournal Article
dc.identifier.doi10.18632/oncotarget.7573
melbourne.affiliation.departmentSurgery (RMH)
melbourne.affiliation.departmentBio21
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.affiliation.facultyAffiliate
melbourne.source.titleOncotarget
melbourne.source.volume7
melbourne.source.issue15
melbourne.source.pages19709-19722
melbourne.identifier.nhmrc433619
dc.rights.licenseCC BY
melbourne.elementsid1042260
melbourne.contributor.authorHanssen, Eric
melbourne.contributor.authorZhu, Hongjian
dc.identifier.eissn1949-2553
melbourne.identifier.fundernameidNHMRC, 433619
melbourne.accessrightsOpen Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record