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dc.contributor.authorMagner, WJ
dc.contributor.authorChang, AC
dc.contributor.authorOwens, J
dc.contributor.authorHong, MJ
dc.contributor.authorBrooks, A
dc.contributor.authorColigan, JE
dc.date.accessioned2021-02-05T01:08:36Z
dc.date.available2021-02-05T01:08:36Z
dc.date.issued2000
dc.identifier.citationMagner, W. J., Chang, A. C., Owens, J., Hong, M. J., Brooks, A. & Coligan, J. E. (2000). Aberrant development of thymocytes in mice lacking laminin-2.. Dev Immunol, 7 (2-4), pp.179-193. https://doi.org/10.1155/2000/90943.
dc.identifier.issn1044-6672
dc.identifier.urihttp://hdl.handle.net/11343/260282
dc.description.abstractIn previous in vitro studies, we proposed a role for the extracellular matrix component, laminin-2, and its integrin receptor, VLA-6, in thymocyte development. The characterization of two dystrophic mouse strains with different defects in laminin-2 allowed us to examine this proposal in vivo. Mice deficient in laminin-2, dy/dy, show a significant reduction in thymus size and number of thymocytes compared to normal littermates. These mice also exhibited apparent alterations of thymic architecture. Examination of the CD4/CD8 populations in dy/dy thymi showed large relative increases in the DN (CD4- CD8-) and SP (CD4+ CD8-, CD4- CD8+) populations and a significant decrease in the DP (CD4+ CD8+) population. Further examination of the DN population for CD44 and CD25 expression showed a remarkable decrease in the more mature pre-T cell populations. Analysis of apoptosis in situ, and by flow cytometry, in dy/dy thymi revealed a significant increase in apoptotic DN thymocytes in the capsule and subcapsular regions. Interestingly, thymocyte development appeared to proceed normally in dystrophic mice expressing a mutant form of laminin-2, dy2J, as well as, in fetal and neonatal dy/dy mice. We propose that laminin-2 plays an active role in thymocyte development by delivering cell survival and differentiation signals at specific stages of development in young adult mice.
dc.languageeng
dc.publisherHindawi Limited
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleAberrant development of thymocytes in mice lacking laminin-2.
dc.typeJournal Article
dc.identifier.doi10.1155/2000/90943
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titleAutoimmunity
melbourne.source.volume7
melbourne.source.issue2-4
melbourne.source.pages179-193
dc.rights.licenseCC BY
melbourne.elementsid1046791
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2276047
melbourne.contributor.authorBrooks, Andrew
melbourne.accessrightsOpen Access


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