Setdb1-mediated H3K9 methylation is enriched on the inactive X and plays a role in its epigenetic silencing
AuthorKeniry, A; Gearing, LJ; Jansz, N; Liu, J; Holik, AZ; Hickey, PF; Kinkel, SA; Moore, DL; Breslin, K; Chen, K; ...
Source TitleEpigenetics and Chromatin
University of Melbourne Author/sBlewitt, Marnie; Kile, Benjamin; Kinkel, Sarah; Sheridan, Julie; Hilton, Douglas; Keniry, Andrew; GEARING, LINDEN; Jansz, Natasha; HOLIK, ALIAKSEI; Hickey, Peter; ...
AffiliationMedical Biology (W.E.H.I.)
School of BioSciences
Document TypeJournal Article
CitationsKeniry, A., Gearing, L. J., Jansz, N., Liu, J., Holik, A. Z., Hickey, P. F., Kinkel, S. A., Moore, D. L., Breslin, K., Chen, K., Liu, R., Phillips, C., Pakusch, M., Biben, C., Sheridan, J. M., Kile, B. T., Carmichael, C., Ritchie, M. E., Hilton, D. J. & Blewitt, M. E. (2016). Setdb1-mediated H3K9 methylation is enriched on the inactive X and plays a role in its epigenetic silencing. EPIGENETICS & CHROMATIN, 9 (1), https://doi.org/10.1186/s13072-016-0064-6.
Access StatusOpen Access
BACKGROUND: The presence of histone 3 lysine 9 (H3K9) methylation on the mouse inactive X chromosome has been controversial over the last 15 years, and the functional role of H3K9 methylation in X chromosome inactivation in any species has remained largely unexplored. RESULTS: Here we report the first genomic analysis of H3K9 di- and tri-methylation on the inactive X: we find they are enriched at the intergenic, gene poor regions of the inactive X, interspersed between H3K27 tri-methylation domains found in the gene dense regions. Although H3K9 methylation is predominantly non-genic, we find that depletion of H3K9 methylation via depletion of H3K9 methyltransferase Set domain bifurcated 1 (Setdb1) during the establishment of X inactivation, results in failure of silencing for around 150 genes on the inactive X. By contrast, we find a very minor role for Setdb1-mediated H3K9 methylation once X inactivation is fully established. In addition to failed gene silencing, we observed a specific failure to silence X-linked long-terminal repeat class repetitive elements. CONCLUSIONS: Here we have shown that H3K9 methylation clearly marks the murine inactive X chromosome. The role of this mark is most apparent during the establishment phase of gene silencing, with a more muted effect on maintenance of the silent state. Based on our data, we hypothesise that Setdb1-mediated H3K9 methylation plays a role in epigenetic silencing of the inactive X via silencing of the repeats, which itself facilitates gene silencing through alterations to the conformation of the whole inactive X chromosome.
- Click on "Export Reference in RIS Format" and choose "open with... Endnote".
- Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References