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dc.contributor.authorJouiaei, M
dc.contributor.authorCasewell, NR
dc.contributor.authorYanagihara, AA
dc.contributor.authorNouwens, A
dc.contributor.authorCribb, BW
dc.contributor.authorWhitehead, D
dc.contributor.authorJackson, TNW
dc.contributor.authorAli, SA
dc.contributor.authorWagstaff, SC
dc.contributor.authorKoludarov, I
dc.contributor.authorAlewood, P
dc.contributor.authorHansen, J
dc.contributor.authorFry, BG
dc.date.accessioned2021-02-05T01:28:37Z
dc.date.available2021-02-05T01:28:37Z
dc.date.issued2015-03-01
dc.identifierpii: toxins7030936
dc.identifier.citationJouiaei, M., Casewell, N. R., Yanagihara, A. A., Nouwens, A., Cribb, B. W., Whitehead, D., Jackson, T. N. W., Ali, S. A., Wagstaff, S. C., Koludarov, I., Alewood, P., Hansen, J. & Fry, B. G. (2015). Firing the Sting: Chemically Induced Discharge of Cnidae Reveals Novel Proteins and Peptides from Box Jellyfish (Chironex fleckeri) Venom. TOXINS, 7 (3), pp.936-950. https://doi.org/10.3390/toxins7030936.
dc.identifier.issn2072-6651
dc.identifier.urihttp://hdl.handle.net/11343/260412
dc.description.abstractCnidarian venom research has lagged behind other toxinological fields due to technical difficulties in recovery of the complex venom from the microscopic nematocysts. Here we report a newly developed rapid, repeatable and cost effective technique of venom preparation, using ethanol to induce nematocyst discharge and to recover venom contents in one step. Our model species was the Australian box jellyfish (Chironex fleckeri), which has a notable impact on public health. By utilizing scanning electron microscopy and light microscopy, we examined nematocyst external morphology before and after ethanol treatment and verified nematocyst discharge. Further, to investigate nematocyst content or "venom" recovery, we utilized both top-down and bottom-up transcriptomics-proteomics approaches and compared the proteome profile of this new ethanol recovery based method to a previously reported high activity and recovery protocol, based upon density purified intact cnidae and pressure induced disruption. In addition to recovering previously characterized box jellyfish toxins, including CfTX-A/B and CfTX-1, we recovered putative metalloproteases and novel expression of a small serine protease inhibitor. This study not only reveals a much more complex toxin profile of Australian box jellyfish venom but also suggests that ethanol extraction method could augment future cnidarian venom proteomics research efforts.
dc.languageEnglish
dc.publisherMDPI AG
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleFiring the Sting: Chemically Induced Discharge of Cnidae Reveals Novel Proteins and Peptides from Box Jellyfish (Chironex fleckeri) Venom
dc.typeJournal Article
dc.identifier.doi10.3390/toxins7030936
melbourne.affiliation.departmentBiochemistry and Molecular Biology
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titleToxins
melbourne.source.volume7
melbourne.source.issue3
melbourne.source.pages936-950
dc.rights.licenseCC BY
melbourne.elementsid978939
melbourne.contributor.authorJackson, Timothy
dc.identifier.eissn2072-6651
melbourne.accessrightsOpen Access


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