Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus.

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    Author
    Christo, SN; Diener, KR; Nordon, RE; Brown, MP; Griesser, HJ; Vasilev, K; Christo, FC; Hayball, JD
    Date
    2015-01-14
    Source Title
    Scientific Reports
    Publisher
    Springer Science and Business Media LLC
    University of Melbourne Author/s
    Christo, Susan
    Affiliation
    Microbiology and Immunology
    Metadata
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    Document Type
    Journal Article
    Citations
    Christo, S. N., Diener, K. R., Nordon, R. E., Brown, M. P., Griesser, H. J., Vasilev, K., Christo, F. C. & Hayball, J. D. (2015). Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus.. Sci Rep, 5 (1), pp.7760-. https://doi.org/10.1038/srep07760.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/260445
    DOI
    10.1038/srep07760
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4293621
    Abstract
    The capture and activation of individual T cells on functionalised surfaces enables real-time analyses of the magnitude and rhythm of intracellular calcium release. Application of Haarlet transformations generate a calcium flux 'threshold', with the frequency of the 'threshold crossings' correlating with the strength of the original T cell stimulus. These findings represent a new method to evaluate graduations in T cell activation in real time, and at a single-cell level.

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