Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus.

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    Author
    Christo, SN; Diener, KR; Nordon, RE; Brown, MP; Griesser, HJ; Vasilev, K; Christo, FC; Hayball, JD
    Date
    2015-01-14
    Source Title
    Scientific Reports
    Publisher
    Springer Science and Business Media LLC
    University of Melbourne Author/s
    Christo, Susan
    Affiliation
    Microbiology and Immunology
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Christo, S. N., Diener, K. R., Nordon, R. E., Brown, M. P., Griesser, H. J., Vasilev, K., Christo, F. C. & Hayball, J. D. (2015). Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus.. Sci Rep, 5 (1), pp.7760-. https://doi.org/10.1038/srep07760.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/260445
    DOI
    10.1038/srep07760
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4293621
    Abstract
    The capture and activation of individual T cells on functionalised surfaces enables real-time analyses of the magnitude and rhythm of intracellular calcium release. Application of Haarlet transformations generate a calcium flux 'threshold', with the frequency of the 'threshold crossings' correlating with the strength of the original T cell stimulus. These findings represent a new method to evaluate graduations in T cell activation in real time, and at a single-cell level.

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