Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus.

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Author
Christo, SN; Diener, KR; Nordon, RE; Brown, MP; Griesser, HJ; Vasilev, K; Christo, FC; Hayball, JDDate
2015-01-14Source Title
Scientific ReportsPublisher
Springer Science and Business Media LLCUniversity of Melbourne Author/s
Christo, SusanAffiliation
Microbiology and ImmunologyMetadata
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Journal ArticleCitations
Christo, S. N., Diener, K. R., Nordon, R. E., Brown, M. P., Griesser, H. J., Vasilev, K., Christo, F. C. & Hayball, J. D. (2015). Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus.. Sci Rep, 5 (1), pp.7760-. https://doi.org/10.1038/srep07760.Access Status
Open AccessOpen Access at PMC
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4293621Abstract
The capture and activation of individual T cells on functionalised surfaces enables real-time analyses of the magnitude and rhythm of intracellular calcium release. Application of Haarlet transformations generate a calcium flux 'threshold', with the frequency of the 'threshold crossings' correlating with the strength of the original T cell stimulus. These findings represent a new method to evaluate graduations in T cell activation in real time, and at a single-cell level.
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