Identification of bacteriophage-encoded anti-sRNAs in pathogenic Escherichia coli.
AuthorTree, JJ; Granneman, S; McAteer, SP; Tollervey, D; Gally, DL
Source TitleMolecular Cell
University of Melbourne Author/sTREE, JAI
AffiliationMicrobiology and Immunology
Document TypeJournal Article
CitationsTree, J. J., Granneman, S., McAteer, S. P., Tollervey, D. & Gally, D. L. (2014). Identification of bacteriophage-encoded anti-sRNAs in pathogenic Escherichia coli.. Mol Cell, 55 (2), pp.199-213. https://doi.org/10.1016/j.molcel.2014.05.006.
Access StatusOpen Access
Open Access at PMChttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4104026
In bacteria, Hfq is a core RNA chaperone that catalyzes the interaction of mRNAs with regulatory small RNAs (sRNAs). To determine in vivo RNA sequence requirements for Hfq interactions, and to study riboregulation in a bacterial pathogen, Hfq was UV crosslinked to RNAs in enterohemorrhagic Escherichia coli (EHEC). Hfq bound repeated trinucleotide motifs of A-R-N (A-A/G-any nucleotide) often associated with the Shine-Dalgarno translation initiation sequence in mRNAs. These motifs overlapped or were adjacent to the mRNA sequences bound by sRNAs. In consequence, sRNA-mRNA duplex formation will displace Hfq, promoting recycling. Fifty-five sRNAs were identified within bacteriophage-derived regions of the EHEC genome, including some of the most abundant Hfq-interacting sRNAs. One of these (AgvB) antagonized the function of the core genome regulatory sRNA, GcvB, by mimicking its mRNA substrate sequence. This bacteriophage-encoded "anti-sRNA" provided EHEC with a growth advantage specifically in bovine rectal mucus recovered from its primary colonization site in cattle.
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