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dc.contributor.authorTree, JJ
dc.contributor.authorGranneman, S
dc.contributor.authorMcAteer, SP
dc.contributor.authorTollervey, D
dc.contributor.authorGally, DL
dc.date.accessioned2021-02-05T01:34:17Z
dc.date.available2021-02-05T01:34:17Z
dc.date.issued2014-07-17
dc.identifierpii: S1097-2765(14)00400-6
dc.identifier.citationTree, J. J., Granneman, S., McAteer, S. P., Tollervey, D. & Gally, D. L. (2014). Identification of bacteriophage-encoded anti-sRNAs in pathogenic Escherichia coli.. Mol Cell, 55 (2), pp.199-213. https://doi.org/10.1016/j.molcel.2014.05.006.
dc.identifier.issn1097-2765
dc.identifier.urihttp://hdl.handle.net/11343/260447
dc.description.abstractIn bacteria, Hfq is a core RNA chaperone that catalyzes the interaction of mRNAs with regulatory small RNAs (sRNAs). To determine in vivo RNA sequence requirements for Hfq interactions, and to study riboregulation in a bacterial pathogen, Hfq was UV crosslinked to RNAs in enterohemorrhagic Escherichia coli (EHEC). Hfq bound repeated trinucleotide motifs of A-R-N (A-A/G-any nucleotide) often associated with the Shine-Dalgarno translation initiation sequence in mRNAs. These motifs overlapped or were adjacent to the mRNA sequences bound by sRNAs. In consequence, sRNA-mRNA duplex formation will displace Hfq, promoting recycling. Fifty-five sRNAs were identified within bacteriophage-derived regions of the EHEC genome, including some of the most abundant Hfq-interacting sRNAs. One of these (AgvB) antagonized the function of the core genome regulatory sRNA, GcvB, by mimicking its mRNA substrate sequence. This bacteriophage-encoded "anti-sRNA" provided EHEC with a growth advantage specifically in bovine rectal mucus recovered from its primary colonization site in cattle.
dc.languageeng
dc.publisherElsevier BV
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleIdentification of bacteriophage-encoded anti-sRNAs in pathogenic Escherichia coli.
dc.typeJournal Article
dc.identifier.doi10.1016/j.molcel.2014.05.006
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titleMolecular Cell
melbourne.source.volume55
melbourne.source.issue2
melbourne.source.pages199-213
dc.rights.licenseCC BY
melbourne.elementsid1108124
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4104026
melbourne.contributor.authorTREE, JAI
dc.identifier.eissn1097-4164
melbourne.accessrightsOpen Access


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