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dc.contributor.authorZheng, Y
dc.contributor.authorHe, R
dc.contributor.authorHe, M
dc.contributor.authorGu, X
dc.contributor.authorWang, T
dc.contributor.authorLai, W
dc.contributor.authorPeng, X
dc.contributor.authorYang, G
dc.date.accessioned2021-02-05T01:35:15Z
dc.date.available2021-02-05T01:35:15Z
dc.date.issued2016-01-22
dc.identifierpii: 10.1186/s12879-016-1353-1
dc.identifier.citationZheng, Y., He, R., He, M., Gu, X., Wang, T., Lai, W., Peng, X. & Yang, G. (2016). Characterization of Sarcoptes scabiei cofilin gene and assessment of recombinant cofilin protein as an antigen in indirect-ELISA for diagnosis. BMC INFECTIOUS DISEASES, 16 (1), https://doi.org/10.1186/s12879-016-1353-1.
dc.identifier.issn1471-2334
dc.identifier.urihttp://hdl.handle.net/11343/260454
dc.description.abstractBACKGROUND: Scabies impairs the health of humans and animals and causes heavy economic losses. Traditional diagnostic methods for scabies are inefficient and ineffective, and so far there is no commercial immunodiagnostic or molecular based test for scabies. METHODS: Here, we used recombinant Sarcoptes scabiei cofilin protein as an antigen to establish indirect ELISA. S. scabiei cofilin is highly homologous to Dermatophagoides farinae Der f 31 allergen (90% identity). The S. scabiei cofilin gene was cloned and expressed in Escherichia coli to obtain recombinant protein. Western blotting and fluorescence immunohistochemistry were carried out, and we established an indirect ELISA method and detected 33 serum samples from scabies infected rabbits and 30 serum samples from naïve rabbits. RESULTS: Western blotting demonstrated that S. scabiei cofilin possessed good immunogenicity and fluorescence immunohistochemistry showed the S. scabiei cofilin is widespread in the splanchnic area of mites. In ELISA, a cut-off value of 0.188 was determined to judge experimental positive and negative serum values. Specificity and sensitivity of the ELISA were 87.9 and 83.33%, respectively. CONCLUSIONS: Recombinant S. scabiei cofilin showed potential value as a diagnostic antigen. The ELISA method established could be used in clinical diagnosis and provide experimental information in minimal or asymptomatic infection.
dc.languageEnglish
dc.publisherBMC
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleCharacterization of Sarcoptes scabiei cofilin gene and assessment of recombinant cofilin protein as an antigen in indirect-ELISA for diagnosis
dc.typeJournal Article
dc.identifier.doi10.1186/s12879-016-1353-1
melbourne.affiliation.departmentVeterinary Biosciences
melbourne.affiliation.facultyVeterinary and Agricultural Sciences
melbourne.source.titleBMC Infectious Diseases
melbourne.source.volume16
melbourne.source.issue1
dc.rights.licenseCC BY
melbourne.elementsid1105912
melbourne.contributor.authorWang, Tao
dc.identifier.eissn1471-2334
melbourne.accessrightsOpen Access


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