Plasmodium falciparum Rab1A Localizes to Rhoptries in Schizonts
AuthorMorse, D; Webster, W; Kalanon, M; Langsley, G; McFadden, GI
Source TitlePLoS One
PublisherPUBLIC LIBRARY SCIENCE
AffiliationSchool of BioSciences
Document TypeJournal Article
CitationsMorse, D., Webster, W., Kalanon, M., Langsley, G. & McFadden, G. I. (2016). Plasmodium falciparum Rab1A Localizes to Rhoptries in Schizonts. PLOS ONE, 11 (6), https://doi.org/10.1371/journal.pone.0158174.
Access StatusOpen Access
Over-expression of a GFP-PfRab1A fusion protein in Plasmodium falciparum schizonts produces a punctate pattern of fluorescence typical of rhoptries, secretory organelles involved in host cell invasion. The GFP-positive bodies were purified by a combination of differential and density gradient centrifugation and their protein content determined by MS/MS sequencing. Consistent with the GFP rhoptry-like pattern of transgenic parasites, four of the 19 proteins identified have been previously described to be rhoptry-associated and another four are ER or ER-associated proteins. Confirmation that GFP-PfRab1A decorates rhoptries was obtained by its co-localization with Rap1 and Ron4 in late phase schizonts. We conclude that PfRab1A potentially regulates vesicular traffic from the endoplasmic reticulum to the rhoptries in Apicomplexa parasites.
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