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dc.contributor.authorLam, YY
dc.contributor.authorHa, CWY
dc.contributor.authorCampbell, CR
dc.contributor.authorMitchell, AJ
dc.contributor.authorDinudom, A
dc.contributor.authorOscarsson, J
dc.contributor.authorCook, DI
dc.contributor.authorHunt, NH
dc.contributor.authorCaterson, ID
dc.contributor.authorHolmes, AJ
dc.contributor.authorStorlien, LH
dc.date.accessioned2021-02-12T00:26:56Z
dc.date.available2021-02-12T00:26:56Z
dc.date.issued2012-03-23
dc.identifierpii: PONE-D-11-25550
dc.identifier.citationLam, Y. Y., Ha, C. W. Y., Campbell, C. R., Mitchell, A. J., Dinudom, A., Oscarsson, J., Cook, D. I., Hunt, N. H., Caterson, I. D., Holmes, A. J. & Storlien, L. H. (2012). Increased Gut Permeability and Microbiota Change Associate with Mesenteric Fat Inflammation and Metabolic Dysfunction in Diet-Induced Obese Mice. PLOS ONE, 7 (3), https://doi.org/10.1371/journal.pone.0034233.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11343/260606
dc.description.abstractWe investigated the relationship between gut health, visceral fat dysfunction and metabolic disorders in diet-induced obesity. C57BL/6J mice were fed control or high saturated fat diet (HFD). Circulating glucose, insulin and inflammatory markers were measured. Proximal colon barrier function was assessed by measuring transepithelial resistance and mRNA expression of tight-junction proteins. Gut microbiota profile was determined by 16S rDNA pyrosequencing. Tumor necrosis factor (TNF)-α and interleukin (IL)-6 mRNA levels were measured in proximal colon, adipose tissue and liver using RT-qPCR. Adipose macrophage infiltration (F4/80⁺) was assessed using immunohistochemical staining. HFD mice had a higher insulin/glucose ratio (P = 0.020) and serum levels of serum amyloid A3 (131%; P = 0.008) but reduced circulating adiponectin (64%; P = 0.011). In proximal colon of HFD mice compared to mice fed the control diet, transepithelial resistance and mRNA expression of zona occludens 1 were reduced by 38% (P<0.001) and 40% (P = 0.025) respectively and TNF-α mRNA level was 6.6-fold higher (P = 0.037). HFD reduced Lactobacillus (75%; P<0.001) but increased Oscillibacter (279%; P = 0.004) in fecal microbiota. Correlations were found between abundances of Lactobacillus (r = 0.52; P = 0.013) and Oscillibacter (r = -0.55; P = 0.007) with transepithelial resistance of the proximal colon. HFD increased macrophage infiltration (58%; P = 0.020), TNF-α (2.5-fold, P<0.001) and IL-6 mRNA levels (2.5-fold; P = 0.008) in mesenteric fat. Increased macrophage infiltration in epididymal fat was also observed with HFD feeding (71%; P = 0.006) but neither TNF-α nor IL-6 was altered. Perirenal and subcutaneous adipose tissue showed no signs of inflammation in HFD mice. The current results implicate gut dysfunction, and attendant inflammation of contiguous adipose, as salient features of the metabolic dysregulation of diet-induced obesity.
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleIncreased Gut Permeability and Microbiota Change Associate with Mesenteric Fat Inflammation and Metabolic Dysfunction in Diet-Induced Obese Mice
dc.typeJournal Article
dc.identifier.doi10.1371/journal.pone.0034233
melbourne.affiliation.departmentChemical and Biomolecular Engineering
melbourne.affiliation.facultyEngineering and Information Technology
melbourne.source.titlePLoS One
melbourne.source.volume7
melbourne.source.issue3
dc.rights.licenseCC BY
melbourne.elementsid1079624
melbourne.contributor.authorMitchell, Andrew
dc.identifier.eissn1932-6203
melbourne.accessrightsOpen Access


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