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dc.contributor.authorKhaw, LT
dc.contributor.authorBall, HJ
dc.contributor.authorGolenser, J
dc.contributor.authorCombes, V
dc.contributor.authorGrau, GE
dc.contributor.authorWheway, J
dc.contributor.authorMitchell, AJ
dc.contributor.authorHunt, NH
dc.date.accessioned2021-02-12T00:27:03Z
dc.date.available2021-02-12T00:27:03Z
dc.date.issued2013-07-12
dc.identifierpii: PONE-D-13-05393
dc.identifier.citationKhaw, L. T., Ball, H. J., Golenser, J., Combes, V., Grau, G. E., Wheway, J., Mitchell, A. J. & Hunt, N. H. (2013). Endothelial Cells Potentiate Interferon-gamma Production in a Novel Tripartite Culture Model of Human Cerebral Malaria. PLOS ONE, 8 (7), https://doi.org/10.1371/journal.pone.0069521.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11343/260607
dc.description.abstractWe have established a novel in vitro co-culture system of human brain endothelial cells (HBEC), Plasmodium falciparum parasitised red blood cells (iRBC) and peripheral blood mononuclear cells (PBMC), in order to simulate the chief pathophysiological lesion in cerebral malaria (CM). This approach has revealed a previously unsuspected pro-inflammatory role of the endothelial cell through potentiating the production of interferon (IFN)-γ by PBMC and concurrent reduction of interleukin (IL)-10. The IFN-γ increased the expression of CXCL10 and intercellular adhesion molecule (ICAM)-1, both of which have been shown to be crucial in the pathogenesis of CM. There was a shift in the ratio of IL-10:IFN-γ protein from >1 to <1 in the presence of HBEC, associated with the pro-inflammatory process in this model. For this to occur, a direct contact between PBMC and HBEC, but not PBMC and iRBC, was necessary. These results support HBEC playing an active role in the pathogenesis of CM. Thus, if these findings reflect the pathogenesis of CM, inhibition of HBEC and PBMC interactions might reduce the occurrence, or improve the prognosis, of the condition.
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleEndothelial Cells Potentiate Interferon-gamma Production in a Novel Tripartite Culture Model of Human Cerebral Malaria
dc.typeJournal Article
dc.identifier.doi10.1371/journal.pone.0069521
melbourne.affiliation.departmentChemical and Biomolecular Engineering
melbourne.affiliation.facultyEngineering and Information Technology
melbourne.source.titlePLoS One
melbourne.source.volume8
melbourne.source.issue7
dc.rights.licenseCC BY
melbourne.elementsid1079627
melbourne.contributor.authorMitchell, Andrew
dc.identifier.eissn1932-6203
melbourne.accessrightsOpen Access


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