Show simple item record

dc.contributor.authorPerkins, TT
dc.contributor.authorKingsley, RA
dc.contributor.authorFookes, MC
dc.contributor.authorGardner, PP
dc.contributor.authorJames, KD
dc.contributor.authorYu, L
dc.contributor.authorAssefa, SA
dc.contributor.authorHe, M
dc.contributor.authorCroucher, NJ
dc.contributor.authorPickard, DJ
dc.contributor.authorMaskell, DJ
dc.contributor.authorParkhill, J
dc.contributor.authorChoudhary, J
dc.contributor.authorThomson, NR
dc.contributor.authorDougan, G
dc.date.accessioned2021-02-12T00:30:45Z
dc.date.available2021-02-12T00:30:45Z
dc.date.issued2009-07-01
dc.identifier.citationPerkins, T. T., Kingsley, R. A., Fookes, M. C., Gardner, P. P., James, K. D., Yu, L., Assefa, S. A., He, M., Croucher, N. J., Pickard, D. J., Maskell, D. J., Parkhill, J., Choudhary, J., Thomson, N. R. & Dougan, G. (2009). A Strand-Specific RNA-Seq Analysis of the Transcriptome of the Typhoid Bacillus Salmonella Typhi. PLOS GENETICS, 5 (7), https://doi.org/10.1371/journal.pgen.1000569.
dc.identifier.issn1553-7404
dc.identifier.urihttp://hdl.handle.net/11343/260638
dc.description.abstractHigh-density, strand-specific cDNA sequencing (ssRNA-seq) was used to analyze the transcriptome of Salmonella enterica serovar Typhi (S. Typhi). By mapping sequence data to the entire S. Typhi genome, we analyzed the transcriptome in a strand-specific manner and further defined transcribed regions encoded within prophages, pseudogenes, previously un-annotated, and 3'- or 5'-untranslated regions (UTR). An additional 40 novel candidate non-coding RNAs were identified beyond those previously annotated. Proteomic analysis was combined with transcriptome data to confirm and refine the annotation of a number of hpothetical genes. ssRNA-seq was also combined with microarray and proteome analysis to further define the S. Typhi OmpR regulon and identify novel OmpR regulated transcripts. Thus, ssRNA-seq provides a novel and powerful approach to the characterization of the bacterial transcriptome.
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleA Strand-Specific RNA-Seq Analysis of the Transcriptome of the Typhoid Bacillus Salmonella Typhi
dc.typeJournal Article
dc.identifier.doi10.1371/journal.pgen.1000569
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titlePLoS Genetics
melbourne.source.volume5
melbourne.source.issue7
dc.rights.licenseCC BY
melbourne.elementsid1081003
melbourne.contributor.authorDougan, Gordon
dc.identifier.eissn1553-7404
melbourne.accessrightsOpen Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record