University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Medical School
  • Microbiology & Immunology
  • Microbiology & Immunology - Research Publications
  • View Item
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Medical School
  • Microbiology & Immunology
  • Microbiology & Immunology - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    A structural basis for selection and cross-species reactivity of the semi-invariant NKT cell receptor in CD1d/glycolipid recognition

    Thumbnail
    Citations
    Scopus
    Web of Science
    Altmetric
    93
    90
    Author
    Kjer-Nielsen, L; Borg, NA; Pellicci, DG; Beddoe, T; Kostenko, L; Clements, CS; Williamson, NA; Smyth, MJ; Besra, GS; Reid, HH; ...
    Date
    2006-03-20
    Source Title
    JOURNAL OF EXPERIMENTAL MEDICINE
    Publisher
    ROCKEFELLER UNIV PRESS
    University of Melbourne Author/s
    Kjer-Nielsen, Lars; Pellicci, Daniel; Kostenko, Lyudmila; Williamson, Nicholas; McCluskey, James; Godfrey, Dale; Bharadwaj, Mandvi
    Affiliation
    Microbiology And Immunology
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Kjer-Nielsen, L., Borg, N. A., Pellicci, D. G., Beddoe, T., Kostenko, L., Clements, C. S., Williamson, N. A., Smyth, M. J., Besra, G. S., Reid, H. H., Bharadwaj, M., Godfrey, D. I., Rossjohn, J. & McCluskey, J. (2006). A structural basis for selection and cross-species reactivity of the semi-invariant NKT cell receptor in CD1d/glycolipid recognition. JOURNAL OF EXPERIMENTAL MEDICINE, 203 (3), pp.661-673. https://doi.org/10.1084/jem.20051777.
    Access Status
    Access this item via the Open Access location
    URI
    http://hdl.handle.net/11343/26155
    DOI
    10.1084/jem.20051777
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118261
    Description

    C1 - Journal Articles Refereed

    Abstract
    Little is known regarding the basis for selection of the semi-invariant alphabeta T cell receptor (TCR) expressed by natural killer T (NKT) cells or how this mediates recognition of CD1d-glycolipid complexes. We have determined the structures of two human NKT TCRs that differ in their CDR3beta composition and length. Both TCRs contain a conserved, positively charged pocket at the ligand interface that is lined by residues from the invariant TCR alpha- and semi-invariant beta-chains. The cavity is centrally located and ideally suited to interact with the exposed glycosyl head group of glycolipid antigens. Sequences common to mouse and human invariant NKT TCRs reveal a contiguous conserved "hot spot" that provides a basis for the reactivity of NKT cells across species. Structural and functional data suggest that the CDR3beta loop provides a plasticity mechanism that accommodates recognition of a variety of glycolipid antigens presented by CD1d. We propose a model of NKT TCR-CD1d-glycolipid interaction in which the invariant CDR3alpha loop is predicted to play a major role in determining the inherent bias toward CD1d. The findings define a structural basis for the selection of the semi-invariant alphabeta TCR and the unique antigen specificity of NKT cells.
    Keywords
    Cellular Immunology; Immune System and Allergy

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [45689]
    • Microbiology & Immunology - Research Publications [1555]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors