Show simple item record

dc.contributor.authorCrimeen-Irwin, B
dc.contributor.authorEllis, S
dc.contributor.authorChristiansen, D
dc.contributor.authorLudford-Menting, MJ
dc.contributor.authorMilland, J
dc.contributor.authorLanteri, M
dc.contributor.authorLoveland, BE
dc.contributor.authorGerlier, D
dc.contributor.authorRussell, SM
dc.date.available2014-05-21T19:25:32Z
dc.date.issued2003-11-21
dc.identifierpii: M308261200
dc.identifier.citationCrimeen-Irwin, B., Ellis, S., Christiansen, D., Ludford-Menting, M. J., Milland, J., Lanteri, M., Loveland, B. E., Gerlier, D. & Russell, S. M. (2003). Ligand binding determines whether CD46 is internalized by clathrin-coated pits or macropinocytosis. JOURNAL OF BIOLOGICAL CHEMISTRY, 278 (47), pp.46927-46937. https://doi.org/10.1074/jbc.M308261200.
dc.identifier.issn0021-9258
dc.identifier.urihttp://hdl.handle.net/11343/26265
dc.descriptionC1 - Journal Articles Refereed
dc.description.abstractCD46 is a ubiquitous human cell surface receptor for the complement components C3b and C4b and for various pathogens, including the measles virus and human herpes virus 6. Ligand binding to CD46 affects (i) protection of autologous cells from complement attack by breakdown of complement components, (ii) intracellular signals that affect the regulation of immune cell function, (iii) antigen presentation, and (iv) down-regulation of cell surface CD46. Recent evidence indicates that CD46 signaling can link innate and acquired immune function. The molecular mechanisms for these processes and the importance of intracellular trafficking of the receptor have not yet been elucidated. We demonstrate here that, in nonlymphoid cells, CD46 is constitutively internalized via clathrin-coated pits, traffics to multivesicular bodies, and is recycled to the cell surface. However, cross-linking of CD46 at the cell surface, by either multivalent antibody or by measles virus, induces pseudopodia that engulf the ligand in a process similar to macropinocytosis, and leads to the degradation of cell surface CD46. Thus, we have elucidated two pathways for CD46 internalization, which are regulated by the valence of cross-linking of CD46 and which utilize either clathrin-coated pits or pseudopodial extension. This has important implications for CD46 signaling, antigen presentation, CD46 down-regulation, and engulfment of pathogens.
dc.formatapplication/pdf
dc.languageEnglish
dc.publisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
dc.subjectImmunology not elsewhere classified; Infectious Diseases; Infectious Diseases
dc.titleLigand binding determines whether CD46 is internalized by clathrin-coated pits or macropinocytosis
dc.typeJournal Article
dc.identifier.doi10.1074/jbc.M308261200
melbourne.peerreviewPeer Reviewed
melbourne.affiliationThe University of Melbourne
melbourne.affiliation.departmentPathology
melbourne.source.titleJOURNAL OF BIOLOGICAL CHEMISTRY
melbourne.source.volume278
melbourne.source.issue47
melbourne.source.pages46927-46937
dc.research.coderfcd320299
dc.research.coderfcd321010
dc.research.codeseo1998730101
melbourne.publicationid18725
melbourne.elementsid257297
melbourne.contributor.authorChristiansen, Dale
melbourne.contributor.authorRussell, Sarah
melbourne.contributor.authorMILLAND, JULIE
melbourne.contributor.authorLudford-Menting, Mandy
dc.identifier.eissn1083-351X
melbourne.conference.locationUnited States
melbourne.accessrightsThis item is currently not available from this repository


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record