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dc.contributor.authorConnell, TG
dc.contributor.authorCurtis, N
dc.contributor.authorRanganathan, SC
dc.contributor.authorButtery, JP
dc.date.available2014-05-21T19:25:55Z
dc.date.issued2006-07-01
dc.identifierpii: thx.2005.048033
dc.identifier.citationConnell, T. G., Curtis, N., Ranganathan, S. C. & Buttery, J. P. (2006). Performance of a whole blood interferon gamma assay for detecting latent infection with Mycobacterium tuberculosis in children. THORAX, 61 (7), pp.616-620. https://doi.org/10.1136/thx.2005.048033.
dc.identifier.issn0040-6376
dc.identifier.urihttp://hdl.handle.net/11343/26275
dc.descriptionC1 - Journal Articles Refereed
dc.description.abstractBACKGROUND: The diagnosis of latent Mycobacterium tuberculosis (MTB) infection with a tuberculin skin test (TST) in children is complicated by the potential influence of prior exposure to Bacille Calmette Geurin (BCG) vaccination or environmental mycobacteria. A whole blood assay has recently been developed to quantitatively measure interferon gamma (IFN-gamma) production by lymphocytes specific to the MTB antigens ESAT-6 and CFP-10, but its use and assessment in children has been limited. A study was undertaken to compare the performance of the whole blood IFN-gamma assay with the TST in diagnosing latent tuberculosis (TB) infection or TB disease in children in routine clinical practice. METHODS: One hundred and six children with a high risk of latent TB infection or TB disease were enrolled in the study. High risk was defined as contact with TB disease, clinical suspicion of TB disease, or recent arrival from an area of high TB prevalence. The whole blood IFN-gamma assay was undertaken in 101 children. RESULTS: Seventeen (17%) of the 101 assays yielded inconclusive results due to failure of positive or negative control assays. There was poor correlation between the whole blood IFN-gamma assay and the TST (kappa statistic 0.3) with 26 (70%) of the 37 children defined as latent TB infection by TST having a negative whole blood IFN-gamma assay. There were no instances of a positive whole blood IFN-gamma assay with a negative TST. Mitogen (positive) control IFN-gamma responses were significantly correlated with age (Spearman's coefficient = 0.53, p<0.001) and, in children with latent TB infection identified by TST, those with a positive IFN-gamma assay were older (median 12.9 v 6.92 years, respectively, p = 0.007). The whole blood IFN-gamma assay was positive in all nine children with TB disease. CONCLUSION: There was poor agreement between the whole blood IFN-gamma assay and TST for the diagnosis of latent TB. The whole blood IFN-gamma assay may have lower sensitivity than the TST in diagnosing TB infection in children. A significant proportion of whole blood IFN-gamma assays fail when used as a screening assay in routine practice.
dc.formatapplication/pdf
dc.languageEnglish
dc.publisherBMJ PUBLISHING GROUP
dc.subjectInfectious Diseases; Paediatrics; Infectious Diseases; Child Health
dc.titlePerformance of a whole blood interferon gamma assay for detecting latent infection with Mycobacterium tuberculosis in children
dc.typeJournal Article
dc.identifier.doi10.1136/thx.2005.048033
melbourne.peerreviewPeer Reviewed
melbourne.affiliationThe University of Melbourne
melbourne.affiliation.departmentPaediatrics Royal Children'S Hospital
melbourne.source.titleTHORAX
melbourne.source.volume61
melbourne.source.issue7
melbourne.source.pages616-620
dc.research.coderfcd321010
dc.research.coderfcd321019
dc.research.codeseo1998730101
dc.research.codeseo1998730204
melbourne.publicationid60568
melbourne.elementsid282766
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2104654
melbourne.contributor.authorCurtis, Richard
melbourne.contributor.authorButtery, Jim
melbourne.contributor.authorRanganathan, Sarath
melbourne.contributor.authorCONNELL, THOMAS JAMES
dc.identifier.eissn1468-3296
melbourne.accessrightsAccess this item via the Open Access location


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