Subtype AE HIV-1 DNA and recombinant Fowlpoxvirus vaccines encoding five shared HIV-1 genes: safety and T cell immunogenicity in macaques
Author
De Rose, R; Chea, S; Dale, CJ; Reece, J; Fernandez, CS; Wilson, KM; Thomson, S; Ramshaw, IA; Coupar, BEH; Boyle, DB; ...Date
2005-03-14Source Title
VACCINEPublisher
ELSEVIER SCI LTDUniversity of Melbourne Author/s
De Rose, Robert; Reece, Jeanette; Fernandez, Caroline; Kent, Stephen; Chea, Socheata; Batten, Caroline; SULLIVAN, MARKAffiliation
Microbiology And ImmunologyMetadata
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Journal ArticleCitations
De Rose, R., Chea, S., Dale, C. J., Reece, J., Fernandez, C. S., Wilson, K. M., Thomson, S., Ramshaw, I. A., Coupar, B. E. H., Boyle, D. B., Sullivan, M. T. & Kent, S. J. (2005). Subtype AE HIV-1 DNA and recombinant Fowlpoxvirus vaccines encoding five shared HIV-1 genes: safety and T cell immunogenicity in macaques. VACCINE, 23 (16), pp.1949-1956. https://doi.org/10.1016/j.vaccine.2004.10.012.Access Status
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C1 - Journal Articles Refereed
Abstract
To induce broad T cell immunity to HIV-1, we evaluated the safety, immunogenicity and dose-response relationship of DNA and recombinant Fowlpoxvirus (rFPV) vaccines encoding five shared HIV subtype AE genes (Gag, Pol, Env, Tat, Rev) in pigtail macaques. The DNA (three doses of either 1 mg or 4.5 mg) and rFPV (a single boost of either 5 x 10(7) or 2 x 10(8) plaque forming units) vaccines were administered intramuscularly without adjuvants. Broadly reactive HIV-specific T cell immunity was stimulated by all doses of the vaccines administered, without significant differences between the high and low doses studied. The vaccines induced both CD4 and CD8 T cell responses to Gag, Pol, Env and Tat/Rev proteins, with CD4 T cell responses being greater in magnitude than CD8 T cell responses. The vaccine-induced T cell responses had significant cross-recognition of heterologous HIV-1 proteins from non-AE HIV-1 subtypes. In conclusion, these subtype AE HIV-1 DNA and rFPV vaccines were safe, induced broad T-cell immunity in macaques, and are suitable for progression into clinical trials.
Keywords
Medical Biochemistry: Nucleic Acids; Infectious DiseasesExport Reference in RIS Format
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