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    Conserved T cell receptor usage in primary and recall responses to an immunodominant influenza virus nucleoprotein epitope

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    Author
    Kedzierska, K; Turner, SJ; Doherty, PC
    Date
    2004-04-06
    Source Title
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
    Publisher
    NATL ACAD SCIENCES
    University of Melbourne Author/s
    Kedzierska, Katherine; Turner, Stephen; Doherty, Peter
    Affiliation
    Microbiology And Immunology
    Metadata
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    Document Type
    Journal Article
    Citations
    Kedzierska, K., Turner, S. J. & Doherty, P. C. (2004). Conserved T cell receptor usage in primary and recall responses to an immunodominant influenza virus nucleoprotein epitope. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 101 (14), pp.4942-4947. https://doi.org/10.1073/pnas.0401279101.
    Access Status
    Access this item via the Open Access location
    URI
    http://hdl.handle.net/11343/26428
    DOI
    10.1073/pnas.0401279101
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC387353
    Description

    C1 - Journal Articles Refereed

    Abstract
    The CD8+ T cell response to the immunodominant DbNP366 epitope has been analyzed sequentially to determine the prevalence and persistence of different T cell antigen receptor (TCR)Vbeta8.3 clonotypes after primary and secondary influenza virus challenge. Based on the length and amino acid sequences of the complementarity-determining region 3 of TCRbeta (CDR3beta) loop and associated Jbeta usage, the same dominant TCRbeta signatures were found in the blood, the spleen, and the site of virus-induced pathology in the infected respiratory tract. Longitudinal analysis demonstrated that TCRbeta prominent in the antigen-driven phase of response persisted into memory and were again expanded after secondary challenge. A proportion of these high-frequency TCRbeta expressed "public" CDR3beta sequences that were detected in every mouse sampled, whereas others were found more than once but were not invariably present. Analysis of N-region nucleotide diversity established that as many as 10 different nucleic acid sequences (maximum of four "nucleotypes" in any one mouse) could encode a single public TCRbeta amino acid sequence. Conversely, whereas some of the unique, "private" TCRbeta achieved a substantial clone size, they were always specified by a single nucleotype. Although there is a strong stochastic element in this response, the public TCRbeta seem to represent a "best fit" for this immunodominant epitope, are selected preferentially from the naive TCR repertoire, and assume even greater prominence after secondary challenge.
    Keywords
    Cellular Immunology; Immune System and Allergy

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