Conserved T cell receptor usage in primary and recall responses to an immunodominant influenza virus nucleoprotein epitope
Author
Kedzierska, K; Turner, SJ; Doherty, PCDate
2004-04-06Source Title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICAPublisher
NATL ACAD SCIENCESAffiliation
Microbiology And ImmunologyMetadata
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Journal ArticleCitations
Kedzierska, K., Turner, S. J. & Doherty, P. C. (2004). Conserved T cell receptor usage in primary and recall responses to an immunodominant influenza virus nucleoprotein epitope. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 101 (14), pp.4942-4947. https://doi.org/10.1073/pnas.0401279101.Access Status
Access this item via the Open Access locationOpen Access at PMC
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC387353Description
C1 - Journal Articles Refereed
Abstract
The CD8+ T cell response to the immunodominant DbNP366 epitope has been analyzed sequentially to determine the prevalence and persistence of different T cell antigen receptor (TCR)Vbeta8.3 clonotypes after primary and secondary influenza virus challenge. Based on the length and amino acid sequences of the complementarity-determining region 3 of TCRbeta (CDR3beta) loop and associated Jbeta usage, the same dominant TCRbeta signatures were found in the blood, the spleen, and the site of virus-induced pathology in the infected respiratory tract. Longitudinal analysis demonstrated that TCRbeta prominent in the antigen-driven phase of response persisted into memory and were again expanded after secondary challenge. A proportion of these high-frequency TCRbeta expressed "public" CDR3beta sequences that were detected in every mouse sampled, whereas others were found more than once but were not invariably present. Analysis of N-region nucleotide diversity established that as many as 10 different nucleic acid sequences (maximum of four "nucleotypes" in any one mouse) could encode a single public TCRbeta amino acid sequence. Conversely, whereas some of the unique, "private" TCRbeta achieved a substantial clone size, they were always specified by a single nucleotype. Although there is a strong stochastic element in this response, the public TCRbeta seem to represent a "best fit" for this immunodominant epitope, are selected preferentially from the naive TCR repertoire, and assume even greater prominence after secondary challenge.
Keywords
Cellular Immunology; Immune System and AllergyExport Reference in RIS Format
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