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dc.contributor.authorKedzierska, K
dc.contributor.authorLa Gruta, NL
dc.contributor.authorDavenport, MP
dc.contributor.authorTurner, SJ
dc.contributor.authorDoherty, PC
dc.date.available2014-05-21T19:32:23Z
dc.date.issued2005-08-09
dc.identifierpii: 0504851102
dc.identifier.citationKedzierska, K., La Gruta, N. L., Davenport, M. P., Turner, S. J. & Doherty, P. C. (2005). Contribution of T cell receptor affinity to overall avidity for virus-specific CD8(+) T cell responses. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 102 (32), pp.11432-11437. https://doi.org/10.1073/pnas.0504851102.
dc.identifier.issn0027-8424
dc.identifier.urihttp://hdl.handle.net/11343/26430
dc.descriptionC1 - Journal Articles Refereed
dc.description.abstractPrior analysis has characterized the clonal characteristics of effector CD8(+) T cells specific for the prominent influenza A virus nucleoprotein (NP) and acid polymerase (PA) peptides presented by H2D(b). Using a single-cell approach and determination of CDR3beta profiles, a limited, predominantly "public" repertoire was found for CD8(+)D(b)NP(366)(+)Vbeta8.3+ cells, whereas diverse and "private" T cell antigen receptor (TCR)beta clonotypes were typical of the CD8(+)D(b)PA(224)(+)Vbeta7+ response. This single-cell approach has now been used to relate the contributions of particular clonotypes (or affinities) to high-avidity TCRs, as defined by binding under conditions of limiting tetramer availability. At least by the measure of CDR3beta usage, no difference could be found between total and high-avidity populations in the spectrum of TCR-pMHC affinities throughout the limited, and relatively public, CD8(+)D(b)NP(366)(+)Vbeta8.3+ populations. Conversely, the more even (by clone size), diverse, and private CD8(+)D(b)PA(224)(+)Vbeta7+ response was characterized by the clear partitioning of the largest T cell clones in the high-avidity compartment. These results suggest that the relatively constrained CD8(+)D(b)NP(366)(+)Vbeta8.3+ set utilizes a relatively narrow range of affinities, whereas the broader CD8(+)D(b)PA(224)(+)Vbeta7+ response is induced at a range of TCR-pMHC affinities. Thus, whereas TCR sequence (or affinity) appears to contribute substantially to the avidity profile of diverse virus-specific CD8+ populations, other mechanisms may be prominent where the TCR spectrum is more limited.
dc.formatapplication/pdf
dc.languageEnglish
dc.publisherNATL ACAD SCIENCES
dc.subjectCellular Immunology; Immune System and Allergy
dc.titleContribution of T cell receptor affinity to overall avidity for virus-specific CD8(+) T cell responses
dc.typeJournal Article
dc.identifier.doi10.1073/pnas.0504851102
melbourne.peerreviewPeer Reviewed
melbourne.affiliationThe University of Melbourne
melbourne.affiliation.departmentMicrobiology And Immunology
melbourne.source.titlePROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
melbourne.source.volume102
melbourne.source.issue32
melbourne.source.pages11432-11437
dc.research.coderfcd320202
dc.research.codeseo1998730102
melbourne.publicationid39336
melbourne.elementsid269656
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC1183580
melbourne.contributor.authorKedzierska, Katherine
melbourne.contributor.authorLa Gruta, Nicole
melbourne.contributor.authorTurner, Stephen
melbourne.contributor.authorDoherty, Peter
dc.identifier.eissn1091-6490
melbourne.accessrightsAccess this item via the Open Access location


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