The CDR3 regions of an immunodominant T cell receptor dictate the 'energetic landscape' of peptide-MHC recognition
Author
Borg, NA; Ely, LK; Beddoe, T; Macdonald, WA; Reid, HH; Clements, CS; Purcell, AW; Kjer-Nielsen, L; Miles, JJ; Burrows, SR; ...Date
2005-02-01Source Title
NATURE IMMUNOLOGYPublisher
NATURE PUBLISHING GROUPAffiliation
Microbiology And ImmunologyMetadata
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Journal ArticleCitations
Borg, N. A., Ely, L. K., Beddoe, T., Macdonald, W. A., Reid, H. H., Clements, C. S., Purcell, A. W., Kjer-Nielsen, L., Miles, J. J., Burrows, S. R., McCluskey, J. & Rossjohn, J. (2005). The CDR3 regions of an immunodominant T cell receptor dictate the 'energetic landscape' of peptide-MHC recognition. NATURE IMMUNOLOGY, 6 (2), pp.171-180. https://doi.org/10.1038/ni1155.Access Status
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10.1038/ni1155Description
C1 - Journal Articles Refereed
Abstract
The energetic bases of T cell recognition are unclear. Here, we studied the 'energetic landscape' of peptide-major histocompatibility complex (pMHC) recognition by an immunodominant alphabeta T cell receptor (TCR). We quantified and evaluated the effect of natural and systematic substitutions in the complementarity-determining region (CDR) loops on ligand binding in the context of the structural detail of each component of the immunodominant TCR-pMHC complex. The CDR1 and CDR2 loops contributed minimal energy through direct recognition of the antigen and instead had a chief function in stabilizing the ligated CDR3 loops. The underlying energetic basis for recognition lay in the CDR3 loops. Therefore the energetic burden of the CDR loops in the TCR-pMHC interaction is variable among TCRs, reflecting the inherent adaptability of the TCR in ligating different ligands.
Keywords
Cellular Immunology; Immune System and AllergyExport Reference in RIS Format
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