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    Release of proinflammatory cytokines and 8-isoprostane from placenta, adipose tissue, and skeletal muscle from normal pregnant women and women with gestational diabetes mellitus

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    Author
    Lappas, M; Permezel, M; Rice, GE
    Date
    2004-11-01
    Source Title
    JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
    Publisher
    ENDOCRINE SOC
    University of Melbourne Author/s
    Lappas, Martha; Permezel, John; Rice, Gregory
    Affiliation
    Obstetrics And Gynaecology Royal Women'S Hospital/Mercy
    Metadata
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    Document Type
    Journal Article
    Citations
    Lappas, M., Permezel, M. & Rice, G. E. (2004). Release of proinflammatory cytokines and 8-isoprostane from placenta, adipose tissue, and skeletal muscle from normal pregnant women and women with gestational diabetes mellitus. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 89 (11), pp.5627-5633. https://doi.org/10.1210/jc.2003-032097.
    Access Status
    This item is currently not available from this repository
    URI
    http://hdl.handle.net/11343/26479
    DOI
    10.1210/jc.2003-032097
    Description

    C1 - Journal Articles Refereed

    Abstract
    The aim of this study was to 1) profile the basal release of TNF-alpha, IL-6, IL-8, and 8-isoprostane (a marker of oxidative stress); and 2) investigate the effect of stimulation on the release of cytokines from sc adipose tissue and skeletal muscle from normal pregnant women and women with gestational diabetes mellitus (GDM). Placenta, sc adipose tissue, and skeletal muscle were incubated in the absence (control) or presence of lipopolysaccharide (LPS; 10 microg/ml), TNF-alpha (10 ng/ml), IL-6 (10 ng/ml), or IL-8 (10 ng/ml). After an 18-h incubation, the medium was collected, and the release of TNF-alpha, IL-6, IL-8, and 8-isoprostane was quantified by ELISA. In all three tissues, 8-isoprostane release was greater in women with GDM, and stimulation with LPS increased 8-isoprostane release from adipose and skeletal muscle, but not placenta, obtained from women with GDM. However, in tissues obtained from normal pregnant women, LPS stimulation increased 8-isoprostane release in placenta and had no effect in adipose tissue and skeletal muscle. Their was no difference in the release of TNF-alpha, IL-6, and IL-8 from placenta, adipose tissue, and skeletal muscle obtained from normal pregnant women and women with GDM. Stimulation of placenta, adipose tissue, and skeletal muscle with LPS and TNF-alpha resulted in greater release of IL-6 and IL-8, whereas only LPS increased TNF-alpha release from all three tissues. The data presented in this study demonstrate that there is a differential release of 8-isoprostane from fetal (placenta) and maternal (adipose tissue and skeletal muscle) tissues obtained from normal pregnant women and women with GDM. These data are consistent with the hypothesis that oxidative stress may be involved in the progression and/or pathogenesis of GDM.
    Keywords
    Obstetrics and Gynaecology ; Endocrine Organs and Diseases (incl. Diabetes)

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