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dc.contributor.authorLappas, M
dc.contributor.authorPermezel, M
dc.contributor.authorRice, GE
dc.date.available2014-05-21T19:34:26Z
dc.date.issued2004-11-01
dc.identifierpii: 89/11/5627
dc.identifier.citationLappas, M., Permezel, M. & Rice, G. E. (2004). Release of proinflammatory cytokines and 8-isoprostane from placenta, adipose tissue, and skeletal muscle from normal pregnant women and women with gestational diabetes mellitus. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 89 (11), pp.5627-5633. https://doi.org/10.1210/jc.2003-032097.
dc.identifier.issn0021-972X
dc.identifier.urihttp://hdl.handle.net/11343/26479
dc.descriptionC1 - Journal Articles Refereed
dc.description.abstractThe aim of this study was to 1) profile the basal release of TNF-alpha, IL-6, IL-8, and 8-isoprostane (a marker of oxidative stress); and 2) investigate the effect of stimulation on the release of cytokines from sc adipose tissue and skeletal muscle from normal pregnant women and women with gestational diabetes mellitus (GDM). Placenta, sc adipose tissue, and skeletal muscle were incubated in the absence (control) or presence of lipopolysaccharide (LPS; 10 microg/ml), TNF-alpha (10 ng/ml), IL-6 (10 ng/ml), or IL-8 (10 ng/ml). After an 18-h incubation, the medium was collected, and the release of TNF-alpha, IL-6, IL-8, and 8-isoprostane was quantified by ELISA. In all three tissues, 8-isoprostane release was greater in women with GDM, and stimulation with LPS increased 8-isoprostane release from adipose and skeletal muscle, but not placenta, obtained from women with GDM. However, in tissues obtained from normal pregnant women, LPS stimulation increased 8-isoprostane release in placenta and had no effect in adipose tissue and skeletal muscle. Their was no difference in the release of TNF-alpha, IL-6, and IL-8 from placenta, adipose tissue, and skeletal muscle obtained from normal pregnant women and women with GDM. Stimulation of placenta, adipose tissue, and skeletal muscle with LPS and TNF-alpha resulted in greater release of IL-6 and IL-8, whereas only LPS increased TNF-alpha release from all three tissues. The data presented in this study demonstrate that there is a differential release of 8-isoprostane from fetal (placenta) and maternal (adipose tissue and skeletal muscle) tissues obtained from normal pregnant women and women with GDM. These data are consistent with the hypothesis that oxidative stress may be involved in the progression and/or pathogenesis of GDM.
dc.formatapplication/pdf
dc.languageEnglish
dc.publisherENDOCRINE SOC
dc.subjectObstetrics and Gynaecology ; Endocrine Organs and Diseases (incl. Diabetes)
dc.titleRelease of proinflammatory cytokines and 8-isoprostane from placenta, adipose tissue, and skeletal muscle from normal pregnant women and women with gestational diabetes mellitus
dc.typeJournal Article
dc.identifier.doi10.1210/jc.2003-032097
melbourne.peerreviewPeer Reviewed
melbourne.affiliationThe University of Melbourne
melbourne.affiliation.departmentObstetrics And Gynaecology Royal Women'S Hospital/Mercy
melbourne.source.titleJOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
melbourne.source.volume89
melbourne.source.issue11
melbourne.source.pages5627-5633
dc.research.coderfcd321014
dc.research.codeseo1998730105
melbourne.publicationid30682
melbourne.elementsid264890
melbourne.contributor.authorLappas, Martha
melbourne.contributor.authorPermezel, John
melbourne.contributor.authorRice, Gregory
dc.identifier.eissn1945-7197
melbourne.accessrightsThis item is currently not available from this repository


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