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    Direct visualization and characterization of erythrocyte flow in human retinal capillaries

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    57
    Author
    Bedggood, P; Metha, A
    Date
    2012-12-01
    Source Title
    Biomedical Optics Express
    Publisher
    OPTICAL SOC AMER
    University of Melbourne Author/s
    Bedggood, Phillip; Metha, Andrew
    Affiliation
    Optometry and Vision Sciences
    Metadata
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    Document Type
    Journal Article
    Citations
    Bedggood, P. & Metha, A. (2012). Direct visualization and characterization of erythrocyte flow in human retinal capillaries. BIOMEDICAL OPTICS EXPRESS, 3 (12), https://doi.org/10.1364/BOE.3.003264.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/264955
    DOI
    10.1364/BOE.3.003264
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521302
    ARC Grant code
    ARC/DE120101931
    ARC/DP0984649
    Abstract
    Imaging the retinal vasculature offers a surrogate view of systemic vascular health, allowing noninvasive and longitudinal assessment of vascular pathology. The earliest anomalies in vascular disease arise in the microvasculature, however current imaging methods lack the spatiotemporal resolution to track blood flow at the capillary level. We report here on novel imaging technology that allows direct, noninvasive optical imaging of erythrocyte flow in human retinal capillaries. This was made possible using adaptive optics for high spatial resolution (1.5 μm), sCMOS camera technology for high temporal resolution (460 fps), and tunable wavebands from a broadband laser for maximal erythrocyte contrast. Particle image velocimetry on our data sequences was used to quantify flow. We observed marked spatiotemporal variability in velocity, which ranged from 0.3 to 3.3 mm/s, and changed by up to a factor of 4 in a given capillary during the 130 ms imaging period. Both mean and standard deviation across the imaged capillary network varied markedly with time, yet their ratio remained a relatively constant parameter (0.50 ± 0.056). Our observations concur with previous work using less direct methods, validating this as an investigative tool for the study of microvascular disease in humans.

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