Dissecting apicoplast targeting in the malaria parasite Plasmodium falciparum
AuthorFoth, BJ; Ralph, SA; Tonkin, CJ; Struck, NS; Fraunholz, M; Roos, DS; Cowman, AF; McFadden, GI
PublisherAMER ASSOC ADVANCEMENT SCIENCE
University of Melbourne Author/sRALPH, STUART A; TONKIN, CHRISTOPHER JOHN; McFadden, Geoffrey; Ralph, Stuart; Cowman, Alan; FOTH, BERNARDO JAVIER; STRUCK, NICOLE
Document TypeJournal Article
CitationsFoth, B. J., Ralph, S. A., Tonkin, C. J., Struck, N. S., Fraunholz, M., Roos, D. S., Cowman, A. F. & McFadden, G. I. (2003). Dissecting apicoplast targeting in the malaria parasite Plasmodium falciparum. SCIENCE, 299 (5607), pp.705-708. https://doi.org/10.1126/science.1078599.
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C1 - Journal Articles Refereed
Transit peptides mediate protein targeting into plastids and are only poorly understood. We extracted amino acid features from transit peptides that target proteins to the relict plastid (apicoplast) of malaria parasites. Based on these amino acid characteristics, we identified 466 putative apicoplast proteins in the Plasmodium falciparum genome. Altering the specific charge characteristics in a model transit peptide by site-directed mutagenesis severely disrupted organellar targeting in vivo. Similarly, putative Hsp70 (DnaK) binding sites present in the transit peptide proved to be important for correct targeting.
KeywordsBotany not elsewhere classified; Biological Sciences
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