Dissecting apicoplast targeting in the malaria parasite Plasmodium falciparum
Author
Foth, BJ; Ralph, SA; Tonkin, CJ; Struck, NS; Fraunholz, M; Roos, DS; Cowman, AF; McFadden, GIDate
2003-01-31Source Title
SCIENCEPublisher
AMER ASSOC ADVANCEMENT SCIENCEUniversity of Melbourne Author/s
RALPH, STUART A; TONKIN, CHRISTOPHER JOHN; McFadden, Geoffrey; Ralph, Stuart; Cowman, Alan; FOTH, BERNARDO JAVIER; STRUCK, NICOLEAffiliation
BotanyMetadata
Show full item recordDocument Type
Journal ArticleCitations
Foth, B. J., Ralph, S. A., Tonkin, C. J., Struck, N. S., Fraunholz, M., Roos, D. S., Cowman, A. F. & McFadden, G. I. (2003). Dissecting apicoplast targeting in the malaria parasite Plasmodium falciparum. SCIENCE, 299 (5607), pp.705-708. https://doi.org/10.1126/science.1078599.Access Status
This item is currently not available from this repositoryDescription
C1 - Journal Articles Refereed
Abstract
Transit peptides mediate protein targeting into plastids and are only poorly understood. We extracted amino acid features from transit peptides that target proteins to the relict plastid (apicoplast) of malaria parasites. Based on these amino acid characteristics, we identified 466 putative apicoplast proteins in the Plasmodium falciparum genome. Altering the specific charge characteristics in a model transit peptide by site-directed mutagenesis severely disrupted organellar targeting in vivo. Similarly, putative Hsp70 (DnaK) binding sites present in the transit peptide proved to be important for correct targeting.
Keywords
Botany not elsewhere classified; Biological SciencesExport Reference in RIS Format
Endnote
- Click on "Export Reference in RIS Format" and choose "open with... Endnote".
Refworks
- Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References