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dc.contributor.authorMintern, JD
dc.contributor.authorMaurice, MM
dc.contributor.authorPloegh, HL
dc.contributor.authorSchott, E
dc.date.available2014-05-21T19:35:43Z
dc.date.issued2004-01-01
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000187427700085&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=d4d813f4571fa7d6246bdc0dfeca3a1c
dc.identifier.citationMintern, J. D., Maurice, M. M., Ploegh, H. L. & Schott, E. (2004). Thymic selection and peripheral activation of CD8 T cells by the same class I MHC/peptide complex. JOURNAL OF IMMUNOLOGY, 172 (1), pp.699-708. https://doi.org/10.4049/jimmunol.172.1.699.
dc.identifier.issn0022-1767
dc.identifier.urihttp://hdl.handle.net/11343/26508
dc.descriptionC1 - Journal Articles Refereed
dc.description.abstractThymic selection is controlled by the interaction between TCR and MHC/peptide. Strength and quality of the signal determine whether thymocytes are selected or deleted. The factors that contribute to this signal remain poorly defined. Here we show that fetal thymic organ cultures (FTOCs) derived from OT-I transgenic mice (the OT-I TCR is restricted by K(b)-SIINFEKL) on a K(b)D(b-/-) background support positive selection, but only when provided with soluble H-2K(b)-SIINFEKL complexes. Selection of CD8 T cells is independent of the valency of the ligand or its capability to coengage CD8 molecules. Both CD8alphaalpha and CD8alphabeta T cells are selected by H-2K(b)-SIINFEKL, but only CD8alphabeta cells are capable of releasing IFN-gamma in response to the same ligand. The alpha(4)beta(7) integrin is up-regulated on postselection thymocytes from FTOCs. After adoptive transfer, FTOC-derived OT-I CD8 T cells divide in response to the agonist peptide SIINFEKL. These results establish that CD8 T cells responsive to their nominal peptide-Ag can be generated in FTOC supplemented with soluble MHC class I molecules equipped with the same peptide.
dc.formatapplication/pdf
dc.languageEnglish
dc.publisherAMER ASSOC IMMUNOLOGISTS
dc.subjectCellular Immunology; Immune System and Allergy
dc.titleThymic selection and peripheral activation of CD8 T cells by the same class I MHC/peptide complex
dc.typeJournal Article
dc.identifier.doi10.4049/jimmunol.172.1.699
melbourne.peerreviewPeer Reviewed
melbourne.affiliationThe University of Melbourne
melbourne.affiliation.departmentMicrobiology And Immunology
melbourne.source.titleJournal of Immunology
melbourne.source.volume172
melbourne.source.issue1
melbourne.source.pages699-708
melbourne.publicationid76203
melbourne.elementsid287516
melbourne.contributor.authorMintern, Justine
dc.identifier.eissn1550-6606
melbourne.accessrightsThis item is currently not available from this repository


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