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dc.contributor.authorRalton, JE
dc.contributor.authorNaderer, T
dc.contributor.authorPiraino, HL
dc.contributor.authorBashtannyk, TA
dc.contributor.authorCallaghan, JM
dc.contributor.authorMcConville, MJ
dc.date.available2014-05-21T19:37:19Z
dc.date.issued2003-10-17
dc.identifierpii: M307660200
dc.identifier.citationRalton, J. E., Naderer, T., Piraino, H. L., Bashtannyk, T. A., Callaghan, J. M. & McConville, M. J. (2003). Evidence that intracellular beta 1-2 mannan is a virulence factor in Leishmania parasites. JOURNAL OF BIOLOGICAL CHEMISTRY, 278 (42), pp.40757-40763. https://doi.org/10.1074/jbc.M307660200.
dc.identifier.issn0021-9258
dc.identifier.urihttp://hdl.handle.net/11343/26546
dc.descriptionC1 - Journal Articles Refereed
dc.description.abstractThe protozoan parasite Leishmania mexicana proliferates within macrophage phagolysosomes in the mammalian host. In this study we provide evidence that a novel class of intracellular beta1-2 mannan oligosaccharides is important for parasite survival in host macrophages. Mannan (degree of polymerization 4-40) is expressed at low levels in non-pathogenic promastigote stages but constitutes 80 and 90% of the cellular carbohydrate in the two developmental stages that infect macrophages, non-dividing promastigotes, and lesion-derived amastigotes, respectively. Mannan is catabolized when parasites are starved of glucose, suggesting a reserve function, and developmental stages having low mannan levels or L. mexicana GDPMP mutants lacking all mannose molecules are highly sensitive to glucose starvation. Environmental stresses, such as mild heat shock or the heat shock protein-90 inhibitor, geldanamycin, that trigger the differentiation of promastigotes to amastigotes, result in a 10-25-fold increase in mannan levels. Developmental stages with low mannan levels or L. mexicana mutants lacking mannan do not survive heat shock and are unable to differentiate to amastigotes or infect macrophages in vitro. In contrast, a L. mexicana mutant deficient only in components of the mannose-rich surface glycocalyx differentiates normally and infects macrophages in vitro. Collectively, these data provide strong evidence that mannan accumulation is important for parasite differentiation and survival in macrophages.
dc.formatapplication/pdf
dc.languageEnglish
dc.publisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
dc.subjectBiochemistry and Cell Biology not elsewhere classified ; Biological Sciences
dc.titleEvidence that intracellular beta 1-2 mannan is a virulence factor in Leishmania parasites
dc.typeJournal Article
dc.identifier.doi10.1074/jbc.M307660200
melbourne.peerreviewPeer Reviewed
melbourne.affiliationThe University of Melbourne
melbourne.affiliation.departmentBiochemistry And Molecular Biology
melbourne.source.titleJOURNAL OF BIOLOGICAL CHEMISTRY
melbourne.source.volume278
melbourne.source.issue42
melbourne.source.pages40757-40763
dc.research.coderfcd270199
dc.research.codeseo1998780105
melbourne.publicationid19492
melbourne.elementsid257923
melbourne.contributor.authorRalton, Julie
melbourne.contributor.authorMcConville, Malcolm
melbourne.contributor.authorCALLAGHAN, JUDITH
melbourne.contributor.authorNADERER, THOMAS
melbourne.contributor.authorPIRAINO, HELENA LOUISE
melbourne.contributor.authorPuhalovich, Tanya
dc.identifier.eissn1083-351X
melbourne.accessrightsThis item is currently not available from this repository


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