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dc.contributor.authorBrown, SA
dc.contributor.authorStambas, J
dc.contributor.authorZhan, XY
dc.contributor.authorSlobod, KS
dc.contributor.authorColeclough, C
dc.contributor.authorZirkel, A
dc.contributor.authorSurman, S
dc.contributor.authorWhite, SW
dc.contributor.authorDoherty, PC
dc.contributor.authorHurwitz, JL
dc.date.available2014-05-21T19:38:50Z
dc.date.issued2003-10-15
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000185866100030&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=d4d813f4571fa7d6246bdc0dfeca3a1c
dc.identifier.citationBrown, S. A., Stambas, J., Zhan, X. Y., Slobod, K. S., Coleclough, C., Zirkel, A., Surman, S., White, S. W., Doherty, P. C. & Hurwitz, J. L. (2003). Clustering of Th cell epitopes on exposed regions of HIV envelope despite defects in antibody activity. JOURNAL OF IMMUNOLOGY, 171 (8), pp.4140-4148. https://doi.org/10.4049/jimmunol.171.8.4140.
dc.identifier.issn0022-1767
dc.identifier.urihttp://hdl.handle.net/11343/26580
dc.descriptionC1 - Journal Articles Refereed
dc.description.abstractA long-standing question in the field of immunology concerns the factors that contribute to Th cell epitope immunodominance. For a number of viral membrane proteins, Th cell epitopes are localized to exposed protein surfaces, often overlapping with Ab binding sites. It has therefore been proposed that Abs on B cell surfaces selectively bind and protect exposed protein fragments during Ag processing, and that this interaction helps to shape the Th cell repertoire. While attractive in concept, this hypothesis has not been thoroughly tested. To test this hypothesis, we have compared Th cell peptide immunodominance in normal C57BL/6 mice with that in C57BL/6( micro MT/ micro MT) mice (lacking normal B cell activity). Animals were first vaccinated with DNA constructs expressing one of three different HIV envelope proteins, after which the CD4(+) T cell response profiles were characterized toward overlapping peptides using an IFN-gamma ELISPOT assay. We found a striking similarity between the peptide response profiles in the two mouse strains. Profiles also matched those of previous experiments in which different envelope vaccination regimens were used. Our results clearly demonstrate that normal Ab activity is not required for the establishment or maintenance of Th peptide immunodominance in the HIV envelope response. To explain the clustering of Th cell epitopes, we propose that localization of peptide on exposed envelope surfaces facilitates proteolytic activity and preferential peptide shuttling through the Ag processing pathway.
dc.formatapplication/pdf
dc.languageEnglish
dc.publisherAMER ASSOC IMMUNOLOGISTS
dc.subjectCellular Immunology; Immune System and Allergy
dc.titleClustering of Th cell epitopes on exposed regions of HIV envelope despite defects in antibody activity
dc.typeJournal Article
dc.identifier.doi10.4049/jimmunol.171.8.4140
melbourne.peerreviewPeer Reviewed
melbourne.affiliationThe University of Melbourne
melbourne.affiliation.departmentMicrobiology And Immunology
melbourne.source.titleJOURNAL OF IMMUNOLOGY
melbourne.source.volume171
melbourne.source.issue8
melbourne.source.pages4140-4148
dc.research.coderfcd320202
dc.research.codeseo1998730102
melbourne.publicationid19225
melbourne.elementsid257683
melbourne.contributor.authorStambas, John
melbourne.contributor.authorDoherty, Peter
dc.identifier.eissn1550-6606
melbourne.accessrightsThis item is currently not available from this repository


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