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dc.contributor.authorBetteridge, T
dc.contributor.authorYang, J
dc.contributor.authorPittard, AJ
dc.contributor.authorPraszkier, J
dc.date.available2014-05-21T19:39:09Z
dc.date.issued2003-04-01
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000181703700017&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=d4d813f4571fa7d6246bdc0dfeca3a1c
dc.identifier.citationBetteridge, T., Yang, J., Pittard, A. J. & Praszkier, J. (2003). Interaction of the initiator protein of an IncB plasmid with its origin of DNA replication. JOURNAL OF BACTERIOLOGY, 185 (7), pp.2210-2218. https://doi.org/10.1128/JB.185.7.2210-2218.2003.
dc.identifier.issn0021-9193
dc.identifier.urihttp://hdl.handle.net/11343/26587
dc.descriptionC1 - Journal Articles Refereed
dc.description.abstractThe replication initiator protein RepA of the IncB plasmid pMU720 was purified and used in DNase I protection assays in vitro. RepA protected a 68-bp region of the origin of replication of pMU720. This region, which lies immediately downstream of the DnaA box, contains four copies of the sequence motif 5'AANCNGCAA3'. Mutational analyses identified this sequence as the binding site specifically recognized by RepA (the RepA box). Binding of RepA to the RepA boxes was ordered and sequential, with the box closest to the DnaA binding site (box 1) occupied first and the most distant boxes (boxes 3 and 4) occupied last. However, only boxes 1, 2, and 4 were essential for origin activity, with box 3 playing a lesser role. Changing the spacing between box 1 and the other three boxes affected binding of RepA in vitro and origin activity in vivo, indicating that the RepA molecules bound to ori(B) interact with one another.
dc.formatapplication/pdf
dc.languageEnglish
dc.publisherAMER SOC MICROBIOLOGY
dc.subjectBacteriology ; Biological Sciences
dc.titleInteraction of the initiator protein of an IncB plasmid with its origin of DNA replication
dc.typeJournal Article
dc.identifier.doi10.1128/JB.185.7.2210-2218.2003
melbourne.peerreviewPeer Reviewed
melbourne.affiliationThe University of Melbourne
melbourne.affiliation.departmentMicrobiology And Immunology
melbourne.source.titleJournal of Bacteriology
melbourne.source.volume185
melbourne.source.issue7
melbourne.source.pages2210-2218
melbourne.publicationid17954
melbourne.elementsid256771
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC151506
melbourne.contributor.authorYang, Ji
melbourne.contributor.authorPittard, Alfred
melbourne.contributor.authorPRASZKIER, JUDYTA
melbourne.contributor.authorBETTERIDGE, THU
dc.identifier.eissn1098-5530
melbourne.accessrightsAccess this item via the Open Access location


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