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    Pseudoknot-dependent translational coupling in repBA genes of the IncB plasmid pMU720 involves reinitiation

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    Author
    Praszkier, J; Pittard, AJ
    Date
    2002-10-01
    Source Title
    JOURNAL OF BACTERIOLOGY
    Publisher
    AMER SOC MICROBIOLOGY
    University of Melbourne Author/s
    PRASZKIER, JUDYTA; Pittard, Alfred
    Affiliation
    Microbiology And Immunology
    Metadata
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    Document Type
    Journal Article
    Citations
    Praszkier, J. & Pittard, A. J. (2002). Pseudoknot-dependent translational coupling in repBA genes of the IncB plasmid pMU720 involves reinitiation. JOURNAL OF BACTERIOLOGY, 184 (20), pp.5772-5780. https://doi.org/10.1128/JB.184.20.5772-5780.2002.
    Access Status
    Access this item via the Open Access location
    URI
    http://hdl.handle.net/11343/26589
    DOI
    10.1128/JB.184.20.5772-5780.2002
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC139621
    Description

    C1 - Journal Articles Refereed

    Abstract
    Replication of the IncB miniplasmid pMU720 requires synthesis of the replication initiator protein, RepA, whose translation is coupled to that of a leader peptide, RepB. The unusual feature of this system is that translational coupling in repBA has to be activated by the formation of a pseudoknot immediately upstream of the repA Shine-Dalgarno sequence. A small antisense RNA, RNAI, controls replication of pMU720 by interacting with repBA mRNA to inhibit expression of repA both directly, by preventing formation of the pseudoknot, and indirectly, by inhibiting translation of repB. The mechanism of translational coupling in repBA was investigated using the specialized ribosome system, which directs a subpopulation of ribosomes that carry an altered anti-Shine-Dalgarno sequence to translate mRNA molecules whose Shine-Dalgarno sequences have been altered to be complementary to the mutant anti-Shine-Dalgarno sequence. Our data indicate that translation of repA involves reinitiation by the ribosome that has terminated translation of repB. The role of the pseudoknot in this process and its effect on the control of copy number in pMU720 are discussed.
    Keywords
    Gene Expression; Biological Sciences

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