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dc.contributor.authorPraszkier, J
dc.contributor.authorPittard, AJ
dc.date.available2014-05-21T19:39:13Z
dc.date.issued2002-10-01
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000178279900028&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=d4d813f4571fa7d6246bdc0dfeca3a1c
dc.identifier.citationPraszkier, J. & Pittard, A. J. (2002). Pseudoknot-dependent translational coupling in repBA genes of the IncB plasmid pMU720 involves reinitiation. JOURNAL OF BACTERIOLOGY, 184 (20), pp.5772-5780. https://doi.org/10.1128/JB.184.20.5772-5780.2002.
dc.identifier.issn0021-9193
dc.identifier.urihttp://hdl.handle.net/11343/26589
dc.descriptionC1 - Journal Articles Refereed
dc.description.abstractReplication of the IncB miniplasmid pMU720 requires synthesis of the replication initiator protein, RepA, whose translation is coupled to that of a leader peptide, RepB. The unusual feature of this system is that translational coupling in repBA has to be activated by the formation of a pseudoknot immediately upstream of the repA Shine-Dalgarno sequence. A small antisense RNA, RNAI, controls replication of pMU720 by interacting with repBA mRNA to inhibit expression of repA both directly, by preventing formation of the pseudoknot, and indirectly, by inhibiting translation of repB. The mechanism of translational coupling in repBA was investigated using the specialized ribosome system, which directs a subpopulation of ribosomes that carry an altered anti-Shine-Dalgarno sequence to translate mRNA molecules whose Shine-Dalgarno sequences have been altered to be complementary to the mutant anti-Shine-Dalgarno sequence. Our data indicate that translation of repA involves reinitiation by the ribosome that has terminated translation of repB. The role of the pseudoknot in this process and its effect on the control of copy number in pMU720 are discussed.
dc.formatapplication/pdf
dc.languageEnglish
dc.publisherAMER SOC MICROBIOLOGY
dc.subjectGene Expression; Biological Sciences
dc.titlePseudoknot-dependent translational coupling in repBA genes of the IncB plasmid pMU720 involves reinitiation
dc.typeJournal Article
dc.identifier.doi10.1128/JB.184.20.5772-5780.2002
melbourne.peerreviewPeer Reviewed
melbourne.affiliationThe University of Melbourne
melbourne.affiliation.departmentMicrobiology And Immunology
melbourne.source.titleJournal of Bacteriology
melbourne.source.volume184
melbourne.source.issue20
melbourne.source.pages5772-5780
melbourne.publicationid8892
melbourne.elementsid251318
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC139621
melbourne.contributor.authorPRASZKIER, JUDYTA
melbourne.contributor.authorPittard, Alfred
dc.identifier.eissn1098-5530
melbourne.accessrightsAccess this item via the Open Access location


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