Role of RepA and DnaA proteins in the opening of the origin of DNA replication of an IncB plasmid
AuthorBetteridge, T; Yang, J; Pittard, AJ; Praszkier, J
Source TitleJOURNAL OF BACTERIOLOGY
PublisherAMER SOC MICROBIOLOGY
AffiliationMicrobiology And Immunology
Document TypeJournal Article
CitationsBetteridge, T., Yang, J., Pittard, A. J. & Praszkier, J. (2004). Role of RepA and DnaA proteins in the opening of the origin of DNA replication of an IncB plasmid. JOURNAL OF BACTERIOLOGY, 186 (12), pp.3785-3793. https://doi.org/10.1128/JB.186.12.3785-3793.2004.
Access StatusAccess this item via the Open Access location
Open Access at PMChttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC419965
C1 - Journal Articles Refereed
The replication initiator protein RepA of the IncB plasmid pMU720 was shown to induce localized unwinding of its cognate origin of replication in vitro. DnaA, the initiator protein of Escherichia coli, was unable to induce localized unwinding of this origin of replication on its own but enhanced the opening generated by RepA. The opened region lies immediately downstream of the last of the three binding sites for RepA (RepA boxes) and covers one turn of DNA helix. A 6-mer sequence, 5'-TCTTAA-3', which lies within the opened region, was essential for the localized unwinding of the origin in vitro and origin activity in vivo. In addition, efficient unwinding of the origin of replication of pMU720 in vitro required the native positioning of the binding sites for the initiator proteins. Interestingly, binding of RepA to RepA box 1, which is essential for origin activity, was not required for the localized opening of the origin in vitro.
KeywordsGene Expression; Biological Sciences
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