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    Role of RepA and DnaA proteins in the opening of the origin of DNA replication of an IncB plasmid

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    12
    12
    Author
    Betteridge, T; Yang, J; Pittard, AJ; Praszkier, J
    Date
    2004-06-01
    Source Title
    JOURNAL OF BACTERIOLOGY
    Publisher
    AMER SOC MICROBIOLOGY
    University of Melbourne Author/s
    Yang, Ji; Pittard, Alfred; PRASZKIER, JUDYTA; BETTERIDGE, THU
    Affiliation
    Microbiology And Immunology
    Metadata
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    Document Type
    Journal Article
    Citations
    Betteridge, T., Yang, J., Pittard, A. J. & Praszkier, J. (2004). Role of RepA and DnaA proteins in the opening of the origin of DNA replication of an IncB plasmid. JOURNAL OF BACTERIOLOGY, 186 (12), pp.3785-3793. https://doi.org/10.1128/JB.186.12.3785-3793.2004.
    Access Status
    Access this item via the Open Access location
    URI
    http://hdl.handle.net/11343/26590
    DOI
    10.1128/JB.186.12.3785-3793.2004
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC419965
    Description

    C1 - Journal Articles Refereed

    Abstract
    The replication initiator protein RepA of the IncB plasmid pMU720 was shown to induce localized unwinding of its cognate origin of replication in vitro. DnaA, the initiator protein of Escherichia coli, was unable to induce localized unwinding of this origin of replication on its own but enhanced the opening generated by RepA. The opened region lies immediately downstream of the last of the three binding sites for RepA (RepA boxes) and covers one turn of DNA helix. A 6-mer sequence, 5'-TCTTAA-3', which lies within the opened region, was essential for the localized unwinding of the origin in vitro and origin activity in vivo. In addition, efficient unwinding of the origin of replication of pMU720 in vitro required the native positioning of the binding sites for the initiator proteins. Interestingly, binding of RepA to RepA box 1, which is essential for origin activity, was not required for the localized opening of the origin in vitro.
    Keywords
    Gene Expression; Biological Sciences

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