Helicobacter-induced expression of Bcl-X-L in B lymphocytes in the mouse model: A possible step in the development of gastric mucosa-associated lymphoid tissue (MALT) lymphoma
Web of Science
AuthorMorgner, A; Sutton, P; O'Rourke, JL; Enno, A; Dixon, MF; Lee, A
Source TitleInternational Journal of Cancer
University of Melbourne Author/sSutton, Philip
Document TypeJournal Article
CitationsMorgner, A., Sutton, P., O'Rourke, J. L., Enno, A., Dixon, M. F. & Lee, A. (2001). Helicobacter-induced expression of Bcl-X-L in B lymphocytes in the mouse model: A possible step in the development of gastric mucosa-associated lymphoid tissue (MALT) lymphoma. INTERNATIONAL JOURNAL OF CANCER, 92 (5), pp.634-640. https://doi.org/10.1002/1097-0215(20010601)92:5<634::AID-IJC1241>3.3.CO;2-M.
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C1 - Journal Articles Refereed
Primary gastric mucosa-associated lymphoid tissue (MALT) lymphoma may develop from chronic infection with Helicobacter sp. in the mouse model. The mechanisms of pathogenesis remain unclear. Regulation of B-cell proliferation and death are important features to investigate. Proteins encoded by bcl-2 family genes, e.g., Bcl-X(L), regulate apoptosis; and alterations in the expression of these genes can contribute to the development of cancer. Our aim was to determine the role of Bcl-X(L) in B lymphocytes in the development of gastric MALT lymphoma associated with Helicobacter infection using the BALB/c mouse model. We analyzed 37 animals with Helicobacter-associated MALT (n = 25), low-grade MALT lymphoma (n = 10) and high-grade lymphoma (n = 2), investigating the in vivo distribution of Bcl-X(L) in B cells/B-lymphoma cells using immunohistochemical analysis. In vitro cultivation of B cells/B-lymphoma cells was employed to perform RT-PCR analysis of Bcl-X(L) mRNA expression after cell stimulation with Helicobacter antigen. We found significant Bcl-X(L) protein expression in B lymphocytes within MALT and low-grade MALT lymphoma, whereas there was no and minimal expression, respectively, of Bcl-X(L) in the 2 high-grade MALT lymphoma cases. Expression of bcl-X(L) mRNA in B lymphocytes was up-regulated in vitro upon Helicobacter-antigen stimulation and associated with prolonged cell survival. These findings support the hypothesis that Bcl-X(L) plays a role in the pathogenesis of B-cell MALT lymphoma by providing cell-survival signals and by triggering the acquisition of MALT.
KeywordsCellular Immunology; Prevention - Biologicals (e.g. Vaccines)
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