Protective immunity against Helicobacter is characterized by a unique transcriptional signature
AuthorMueller, A; O'Rourke, J; Chu, P; Kim, CC; Sutton, P; Lee, A; Falkow, S
Source TitlePROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
PublisherNATL ACAD SCIENCES
University of Melbourne Author/sSutton, Philip
Document TypeJournal Article
CitationsMueller, A., O'Rourke, J., Chu, P., Kim, C. C., Sutton, P., Lee, A. & Falkow, S. (2003). Protective immunity against Helicobacter is characterized by a unique transcriptional signature. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 100 (21), pp.12289-12294. https://doi.org/10.1073/pnas.1635231100.
Access StatusAccess this item via the Open Access location
Open Access at PMChttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC218751
C1 - Journal Articles Refereed
Immunization with a whole-cell sonicate vaccine of Helicobacter felis in conjunction with cholera toxin as a mucosal adjuvant induces long-term protective immunity in a majority of laboratory mice. We have combined gene expression profiling and immunohistochemical analysis on a set of immunized animals to better understand the mechanism of protection. The stomachs of protected animals exhibited a strikingly different transcriptional profile compared with those of nonprotected or control mice, indicating that vaccination targets the appropriate site and leaves a molecular signature. Among the genes whose up-regulation is significantly correlated with protection are a number of adipocyte-specific factors. These include the fat-cell-specific cytokines adipsin, resistin, and adiponectin and the adipocyte surface marker CD36. Interestingly, potentially protective T and B lymphocytes can be found embedded in the adipose tissue surrounding protected stomachs but never in control or unprotected stomachs. Adipsin-specific immunohistochemical staining of protected stomach sections further revealed molecular cross-talk between adjacent lymphoid and adipose cell populations. We propose a mechanism of protection that involves the effector responses of either or both lymphocyte subclasses as well as the previously unappreciated paracrine functions of adipose tissue surrounding the resident lymphocytes.
KeywordsImmunology ; Biological Sciences
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