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dc.contributor.authorMueller, A
dc.contributor.authorO'Rourke, J
dc.contributor.authorChu, P
dc.contributor.authorKim, CC
dc.contributor.authorSutton, P
dc.contributor.authorLee, A
dc.contributor.authorFalkow, S
dc.date.available2014-05-21T19:40:09Z
dc.date.issued2003-10-14
dc.identifierpii: 1635231100
dc.identifier.citationMueller, A., O'Rourke, J., Chu, P., Kim, C. C., Sutton, P., Lee, A. & Falkow, S. (2003). Protective immunity against Helicobacter is characterized by a unique transcriptional signature. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 100 (21), pp.12289-12294. https://doi.org/10.1073/pnas.1635231100.
dc.identifier.issn0027-8424
dc.identifier.urihttp://hdl.handle.net/11343/26609
dc.descriptionC1 - Journal Articles Refereed
dc.description.abstractImmunization with a whole-cell sonicate vaccine of Helicobacter felis in conjunction with cholera toxin as a mucosal adjuvant induces long-term protective immunity in a majority of laboratory mice. We have combined gene expression profiling and immunohistochemical analysis on a set of immunized animals to better understand the mechanism of protection. The stomachs of protected animals exhibited a strikingly different transcriptional profile compared with those of nonprotected or control mice, indicating that vaccination targets the appropriate site and leaves a molecular signature. Among the genes whose up-regulation is significantly correlated with protection are a number of adipocyte-specific factors. These include the fat-cell-specific cytokines adipsin, resistin, and adiponectin and the adipocyte surface marker CD36. Interestingly, potentially protective T and B lymphocytes can be found embedded in the adipose tissue surrounding protected stomachs but never in control or unprotected stomachs. Adipsin-specific immunohistochemical staining of protected stomach sections further revealed molecular cross-talk between adjacent lymphoid and adipose cell populations. We propose a mechanism of protection that involves the effector responses of either or both lymphocyte subclasses as well as the previously unappreciated paracrine functions of adipose tissue surrounding the resident lymphocytes.
dc.formatapplication/pdf
dc.languageEnglish
dc.publisherNATL ACAD SCIENCES
dc.subjectImmunology ; Biological Sciences
dc.titleProtective immunity against Helicobacter is characterized by a unique transcriptional signature
dc.typeJournal Article
dc.identifier.doi10.1073/pnas.1635231100
melbourne.peerreviewPeer Reviewed
melbourne.affiliationThe University of Melbourne
melbourne.affiliation.departmentVeterinary Science
melbourne.source.titlePROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
melbourne.source.volume100
melbourne.source.issue21
melbourne.source.pages12289-12294
dc.research.coderfcd300504
dc.research.codeseo1998780105
melbourne.publicationid76512
melbourne.elementsid287747
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC218751
melbourne.contributor.authorSutton, Philip
dc.identifier.eissn1091-6490
melbourne.accessrightsAccess this item via the Open Access location


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