dc.contributor.author | Mueller, A | |
dc.contributor.author | O'Rourke, J | |
dc.contributor.author | Chu, P | |
dc.contributor.author | Kim, CC | |
dc.contributor.author | Sutton, P | |
dc.contributor.author | Lee, A | |
dc.contributor.author | Falkow, S | |
dc.date.available | 2014-05-21T19:40:09Z | |
dc.date.issued | 2003-10-14 | |
dc.identifier | pii: 1635231100 | |
dc.identifier.citation | Mueller, A., O'Rourke, J., Chu, P., Kim, C. C., Sutton, P., Lee, A. & Falkow, S. (2003). Protective immunity against Helicobacter is characterized by a unique transcriptional signature. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 100 (21), pp.12289-12294. https://doi.org/10.1073/pnas.1635231100. | |
dc.identifier.issn | 0027-8424 | |
dc.identifier.uri | http://hdl.handle.net/11343/26609 | |
dc.description | C1 - Journal Articles Refereed | |
dc.description.abstract | Immunization with a whole-cell sonicate vaccine of Helicobacter felis in conjunction with cholera toxin as a mucosal adjuvant induces long-term protective immunity in a majority of laboratory mice. We have combined gene expression profiling and immunohistochemical analysis on a set of immunized animals to better understand the mechanism of protection. The stomachs of protected animals exhibited a strikingly different transcriptional profile compared with those of nonprotected or control mice, indicating that vaccination targets the appropriate site and leaves a molecular signature. Among the genes whose up-regulation is significantly correlated with protection are a number of adipocyte-specific factors. These include the fat-cell-specific cytokines adipsin, resistin, and adiponectin and the adipocyte surface marker CD36. Interestingly, potentially protective T and B lymphocytes can be found embedded in the adipose tissue surrounding protected stomachs but never in control or unprotected stomachs. Adipsin-specific immunohistochemical staining of protected stomach sections further revealed molecular cross-talk between adjacent lymphoid and adipose cell populations. We propose a mechanism of protection that involves the effector responses of either or both lymphocyte subclasses as well as the previously unappreciated paracrine functions of adipose tissue surrounding the resident lymphocytes. | |
dc.format | application/pdf | |
dc.language | English | |
dc.publisher | NATL ACAD SCIENCES | |
dc.subject | Immunology ; Biological Sciences | |
dc.title | Protective immunity against Helicobacter is characterized by a unique transcriptional signature | |
dc.type | Journal Article | |
dc.identifier.doi | 10.1073/pnas.1635231100 | |
melbourne.peerreview | Peer Reviewed | |
melbourne.affiliation | The University of Melbourne | |
melbourne.affiliation.department | Veterinary Science | |
melbourne.source.title | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | |
melbourne.source.volume | 100 | |
melbourne.source.issue | 21 | |
melbourne.source.pages | 12289-12294 | |
dc.research.coderfcd | 300504 | |
dc.research.codeseo1998 | 780105 | |
melbourne.publicationid | 76512 | |
melbourne.elementsid | 287747 | |
melbourne.openaccess.pmc | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC218751 | |
melbourne.contributor.author | Sutton, Philip | |
dc.identifier.eissn | 1091-6490 | |
melbourne.accessrights | Access this item via the Open Access location | |